ID | 110107 |
Title Alternative | DNAメチル化は3T3-L1脂肪細胞におけるレプチン発現を抑制する
DNA Methylation Suppresses Leptin Expression in Adipocytes
|
Author |
Tominaga, Ayako
Tokushima University
Nakagawa, Kasumi
Tokushima University
Nishiguchi, Misa
Tokushima University
Sebe, Mayu
Tokushima University
Miyatake, Yumiko
Tokushima University
Kitamura, Tadahiro
Japan Society for the Promotion of Science
Tsutsumi, Rie
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Nakaya, Yutaka
Shikoku Central Hospital
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
|
Keywords | Adipocyte
Leptin
DNA methylation
3T3-L1
obesity
|
Content Type |
Thesis or Dissertation
|
Description | Leptin is a key regulator of energy intake and expenditure. This peptide hormone is expressed in mouse white adipose tissue, but hardly expressed in 3T3-L1 adipocytes. Using bisulfite sequencing, we found that CpG islands in the leptin promoter are highly methylated in 3T3-L1cells. 5-azacytidine, an inhibitor of DNA methyltransferase, markedly increased leptin expression as pre-adipocytes matured into adipocytes. Remarkably, leptin expression was stimulated by insulin in adipocytes derived from precursor cells exposed to 5-azacytidine, but suppressed by thiazolidinedione and dexamethasone. In contrast, adipocytes derived from untreated precursor cells were unresponsive to both 5-azacytidine and hormonal stimuli, although lipid accumulation was sufficient to boost leptin expression in the absence of demethylation. Taken together, the results suggest that leptin expression in 3T3-L1 cells requires DNA demethylation prior to adipogenesis, transcriptional activation during adipogenesis, and lipid accumulation after adipogenesis.
|
Journal Title |
PLOS ONE
|
ISSN | 19326203
|
Publisher | PLOS
|
Volume | 11
|
Issue | 8
|
Start Page | e0160532
|
Published Date | 2016-08-05
|
Remark | 内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201704261022.pdf 論文本文:LID201704261023.pdf 本論文は, 著者Masashi Kurodaの学位論文として提出され, 学位審査・授与の対象となっている。 |
Rights | This is an open access article distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
|
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
|
TextVersion |
ETD
|
MEXT report number | 甲第3087号
|
Diploma Number | 甲栄第239号
|
Granted Date | 2017-03-23
|
Degree Name |
Doctor of Nutritional Science
|
Grantor |
Tokushima University
|
departments |
Medical Sciences
|