ID | 110124 |
Title Alternative | 食餌性リンは大腸炎モデル動物の病態を悪化させる
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Author |
Sugihara, Kohei
Tokushima University
Masuda, Masashi
Tokushima University
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Nakao, Mari
Tokushima University
Abuduli, Maerjianghan
Tokushima University
Imi, Yukiko
Tokushima University
Oda, Naoko
Tokushima University
Okahisa, Toshiya
Tokushima University
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Takeda, Eiji
Tokushima University
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Keywords | Dietary Pi
DSS-induced colitis
proinflammatory cytokine
NF-κB
ROS
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Content Type |
Thesis or Dissertation
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Description | The recent widespread consumption of Western diets and food additives worldwide is associated with excessive inorganic phosphate intake. However, researchers have known little about the impact of dietary phosphate intake on the development of inflammatory bowel disease to date. In this study, we investigated the effects of dietary phosphate on intestinal inflammation in experimental colitis. Sprague-Dawley rats were fed different phosphate diets (0.5%, 1.0% and 1.5% phosphate) with or without dextran sulfate sodium. For in vitro study, the effects of phosphate on proinflammatory cytokine induction and reactive oxygen species production in RAW264.7 macrophage were examined. Dietary phosphate exacerbated intestinal inflammation in experimental colitis in a dose-dependent manner, as assessed by the clinical disease activity score, colon length, and histology. Furthermore, the high phosphate diet increased myeloperoxidase activity and proinflammatory cytokine mRNA expression through the activation of nuclear factor κB in the inflamed colon. In addition, high phosphate loading in RAW264.7 cells directly enhanced reactive oxygen species production and proinflammatory cytokine gene expression. Our results demonstrated that the high phosphate diet exacerbated intestinal inflammation in experimental colitis. These findings have important therapeutic implications for inflammatory bowel disease patients.
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Journal Title |
Journal of Clinical Biochemistry and Nutrition
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ISSN | 18805086
09120009
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NCID | AA10710201
AA12091064
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Publisher | SOCIETY FOR FREE RADICAL RESEARCH JAPAN
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Volume | 61
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Issue | 2
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Start Page | 91
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End Page | 99
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Published Date | 2017-09-01
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Remark | 内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201705191027.pdf 論文本文:k3010_fulltext.pdf 著者の申請により要約(2017-05-19公開)に替えて論文全文を公開(2018-02-15) 本論文は, 著者Kohei Sugiharaの学位論文として提出され, 学位審査・授与の対象となっている。 |
Rights | © 2017 Society for Free Radical Research Japan
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3090号
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Diploma Number | 甲栄第242号
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Granted Date | 2017-03-23
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Degree Name |
Doctor of Nutritional Science
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Grantor |
Tokushima University
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departments |
Medical Sciences
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