ID | 110108 |
Title Alternative | Campylobacter jejuni感染はT-84細胞におけるCFTR活性化によるCl⁻分泌亢進を抑制する
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Author |
Negoro, Sachie
The University of Tokushima
Hatayama, Syo
The University of Tokushima
Sato, Yuri
The University of Tokushima
Matsumoto, Mari
The University of Tokushima
Iba, Hitomi
The University of Tokushima
Aihara, Mutsumi
The University of Tokushima
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Uebansou, Takashi
The University of Tokushima
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Hamada, Yasuhiro
The University of Tokushima
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Nishikawa, Yoshikazu
Osaka City University
Yamasaki, Shinji
Osaka Prefecture University
Mawatari, Kazuaki
The University of Tokushima
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Takahashi, Akira
The University of Tokushima
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Keywords | Campylobacter jejuni
CFTR
diarrhea
short-circuit current
efflux assay
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Content Type |
Thesis or Dissertation
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Description | Campylobacter jejuni causes foodborne disease associated with abdominal pain, gastroenteritis, and diarrhea. These symptoms are induced by bacterial adherence and invasion of host epithelial cells. C. jejuni infection can occur with a low infective dose, suggesting that C. jejuni may have evolved strategies to cope with the bacterial clearance system in the gastrointestinal tract. The mucosa layer is the first line of defense against bacteria. Mucus conditions are maintained by water and anion (especially Cl-) movement. Cystic fibrosis transmembrane conductance regulator (CFTR) is the main Cl- channel transporting Cl- to the lumen. Mutations in CFTR result in dehydrated secreted mucus and bacterial accumulation in the lungs, and recent studies suggest that closely related pathogenic bacteria also may survive in the intestine. However, the relationship between C. jejuni infection and CFTR has been little studied.
Here, we used an 125I- efflux assay and measurement of short-circuit current to measure Cl- secretion in C. jejuni-infected T-84 human intestinal epithelial cells. The basic state of Cl- secretion was unchanged by C. jejuni infection, but CFTR activator was observed to induce Cl- secretion suppressed in C. jejuniinfected T-84 cells. The suppression of activated Cl- secretion was bacterial dose-dependent and duration-dependent. A similar result was observed during infection with other C. jejuni strains. The mechanism of suppression may occur by affecting water movement or mucus condition in the intestinal tract. A failure of mucus barrier function may promote bacterial adhesion or invasion of host intestinal epithelial cells, thereby causing bacterial preservation in the host intestinal tract. |
Journal Title |
Journal of Infection and Chemotherapy
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ISSN | 1341321X
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NCID | AA11057978
AA11627318
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Publisher | Japanese Society of Chemotherapy and The Japanese Association for Infection Disease|Elsevier
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Volume | 20
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Issue | 11
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Start Page | 682
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End Page | 688
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Published Date | 2014-08-06
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Remark | 内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201704261024.pdf 論文本文:LID201704261025.pdf 本論文は, 著者Sachie Negoroの学位論文として提出され, 学位審査・授与の対象となっている。 |
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3089号
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Diploma Number | 甲栄第241号
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Granted Date | 2017-03-23
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Degree Name |
Doctor of Nutritional Science
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Grantor |
Tokushima University
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departments |
Medical Sciences
Bioscience and Bioindustry
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