ID | 109571 |
Title Alternative | プロポフォールによるラット大動脈の弛緩性は年齢により減弱する
Propofol-induced vasodilation and aging
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Author |
Sakai, Yoko
Tokushima University
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Kawahito, Shinji
Tokushima University
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Takaishi, Kazumi
the University of Tokushima
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Kinoshita, Hiroyuki
Aichi Medical University
Hatakeyama, Noboru
Aichi Medical University
Azma, Toshiharu
National Center for Global Health and Medicine
Nakaya, Yutaka
Shikoku Central Hospital
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Kitahata, Hiroshi
the University of Tokushima
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Keywords | propofol
vasorelaxation
rat aorta
aging
endothelium
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Content Type |
Thesis or Dissertation
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Description | Background : Propofol causes vasodilation via endothelium-dependent and -independent mechanisms. Because endothelial function is impaired with aging, the effects of propofol on endothelium-dependent vasodilation might be altered by aging. The aim of this study was thus to determine the effects of aging on vascular responses to propofol. Methods : Young (4-6 weeks old) or adult (16-25 weeks old) rats were anesthetized with sevoflurane. The thoracic aorta was dissected and cut into pieces 3-4mm in length. In some rings, the endothelium was deliberately removed. The ring segment of the aorta was mounted for isometric force recording at a resting tension of 0.5-1.0 g in a 2 ml organ bath, containing Krebs-Ringer bicarbonate buffer. Arteries were precontracted with phenylephrine, and the function of endothelium was confirmed with acetylcholine. Then, we studied the concentration-dependent effects of propofol in endothelium-intact (control group) and -denuded aortic rings (denuded group), as well as those treated with N[ω]-nitro-L-arginine methylester (L-NAME group). Results : Relaxation due to propofol was observed in the control groups of both young and adult rats in a concentration-dependent manner, but the magnitude of relaxation was significantly greater in young rats. In addition, in young rats, relaxation due to propofol was significantly and equally reduced in both L-NAME and denuded groups at all propofol concentrations that we studied (10[-6]-10[-3] M). In adult rats, relaxation due to propofol was quite similar between control and L-NAME groups at all propofol concentrations, whereas it was significantly reduced in the denuded group. Conclusion : These results suggest that endothelium-derived nitric oxide plays an important role in propofol-induced vasodilation in young rats, but not in adult rats.
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Journal Title |
The journal of medical investigation : JMI
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ISSN | 13431420
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NCID | AA11166929
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Volume | 61
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Issue | 3-4
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Start Page | 278
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End Page | 284
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Sort Key | 278
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Published Date | 2014-08
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Remark | 内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201505141001.pdf 論文本文 : LID201505141002.pdf 本論文は,著者Yoko Sakaiの学位論文として提出され,学位審査・授与の対象となっている。 |
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第2791号
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Diploma Number | 甲医第1233号
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Granted Date | 2015-02-26
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
University Hospital
Medical Sciences
Oral Sciences
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