ID | 117828 |
Author |
Shiraki, Taisei
Tokushima University
Hayashi, Takuma
Tokushima University
Ozue, Jotaro
Tokushima University
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Keywords | CRISPR/Cas9
pronephros
transcription factor
Wilms tumor
WT1
Xenopus
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Content Type |
Journal Article
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Description | The Wilms’ tumor suppressor gene, wt1, encodes a zinc finger-containing transcription factor that binds to a GC-rich motif and regulates the transcription of target genes. wt1 was first identified as a tumor suppressor gene in Wilms’ tumor, a pediatric kidney tumor, and has been implicated in normal kidney development. The WT1 protein has transcriptional activation and repression domains and acts as a transcriptional activator or repressor, depending on the target gene and context. In Xenopus, an ortholog of wt1 has been isolated and shown to be expressed in the developing embryonic pronephros. To investigate the role of wt1 in pronephros development in Xenopus embryos, we mutated wt1 by CRISPR/Cas9 and found that the expression of pronephros marker genes was reduced. In reporter assays in which known WT1 binding sequences were placed upstream of the luciferase gene, WT1 activated transcription of the luciferase gene. The injection of wild-type or artificially altered transcriptional activity of wt1 mRNA disrupted the expression of pronephros marker genes in the embryos. These results suggest that the appropriate amounts and activity of WT1 protein are required for normal pronephros development in Xenopus embryos.
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Journal Title |
Journal of Developmental Biology
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ISSN | 22213759
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Publisher | MDPI
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Volume | 10
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Issue | 4
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Start Page | 46
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Published Date | 2022-10-29
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Rights | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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language |
eng
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departments |
Liberal Arts and Sciences
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