ID | 111987 |
Author |
Ikeda, Mizuki
Tokushima University
Deguchi, Junji
Tokushima University
Fukushima, Shota
Tokushima University
Qingyu, Ai
Tokushima University
Katayama, Norihiko
Tokushima University|Tohoku University
Miura, Hajime
Tokushima University
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Keywords | Chlorothalonil
Intracellular Zn2+
Nonprotein thiol
Cytotoxicity
Lymphocyte
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Content Type |
Journal Article
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Description | Chlorothalonil, a polychlorinated aromatic fungicide, is considered non-toxic to small mammals. However, chlorothalonil inactivates sulfhydryl enzymes and depletes cellular glutathione. Chlorothalonil increases intracellular Zn2+ concentration ([Zn2+]i) in mammalian cells possibly because intracellular Zn2+ is released via zinc-thiol/disulfide interchange. The effects of chlorothalonil at sublethal concentrations on the cellular content of nonprotein thiols ([NPT]i) and [Zn2+]i were examined using flow cytometry in rat thymocytes. Low concentrations (0.3–1 μM) of chlorothalonil increased, but high concentrations (3–10 μM) decreased [NPT]i. These effects of chlorothalonil were partly attenuated by an intracellular Zn2+ chelator. Chlorothalonil at 0.3–10 μM increased [Zn2+]i in a concentration-dependent manner, which was largely dependent on the release of intracellular Zn2+. Both the decrease in [NPT]i and increase in [Zn2+]i increase the vulnerability of cells to oxidative stress. Chlorothalonil at 1–10 μM potentiated the cytotoxicity of H2O2 (300 μM). It was also the case for 10 μM pentachloronitrobenzene, but not 10 μM pentachlorophenol. In conclusion, chlorothalonil at low (sublethal) micromolar concentrations is cytotoxic to mammalian cells under oxidative stress.
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Journal Title |
Environmental Toxicology and Pharmacology
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ISSN | 13826689
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NCID | AA1107880X
AA11526671
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Publisher | Elsevier
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Volume | 59
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Start Page | 61
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End Page | 65
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Published Date | 2018-03-08
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Rights | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
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language |
eng
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TextVersion |
Author
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departments |
Integrated Arts and Sciences
Bioscience and Bioindustry
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