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ID 112395
Title Alternative
等張または低張条件下のラット耳下腺腺房細胞におけるα1-アドレナリン受容体誘導性のAQP5細胞内移動機序
Author
Bragiel, Aneta Magdalena Tokushima University
Wang, Di Tokushima University|Fourth Military Medical University
Pieczonka, Tomasz D. Tokushima University
Keywords
aquaporin-5
α1A-adrenoceptor
α1B-adrenoceptor
α1D-adrenoceptor
calcium
protein G kinase
hypotonicity
Content Type
Thesis or Dissertation
Description
Defective cellular trafficking of aquaporin-5 (AQP5) to the apical plasma membrane (APM) in salivary glands is associated with the loss of salivary fluid secretion. To examine mechanisms of α1-adrenoceptor (AR)-induced trafficking of AQP5, immunoconfocal microscopy and Western blot analysis were used to analyze AQP5 localization in parotid tissues stimulated with phenylephrine under different osmolality. Phenylephrine-induced trafficking of AQP5 to the APM and lateral plasma membrane (LPM) was mediated via the α1A-AR subtype, but not the α1B- and α1D-AR subtypes. Phenylephrine-induced trafficking of AQP5 was inhibited by ODQ and KT5823, inhibitors of nitric oxide (NO)-stimulated guanylcyclase (GC) and protein kinase (PK) G, respectively, indicating the involvement of the NO/ soluble (c) GC/PKG signaling pathway. Under isotonic conditions, phenylephrine-induced trafficking was inhibited by La3+, implying the participation of store-operated Ca2+ channel. Under hypotonic conditions, phenylephrine-induced trafficking of AQP5 to the APM was higher than that under isotonic conditions. Under non-stimulated conditions, hypotonicity-induced trafficking of AQP5 to the APM was inhibited by ruthenium red and La3+, suggesting the involvement of extracellular Ca2+ entry. Thus, α1A-AR activation induced the trafficking of AQP5 to the APM and LPM via the Ca2+/ cyclic guanosine monophosphate (cGMP)/PKG signaling pathway, which is associated with store-operated Ca2+ entry.
Journal Title
International Journal of Molecular Sciences
ISSN
14220067
16616596
NCID
AA12038549
Publisher
MDPI
Volume
17
Issue
7
Start Page
1022
Published Date
2016-06-28
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704251013.pdf
審査要旨:LID201704251014.pdf
論文本文:LID201704251015.pdf
本論文は,著者Aneta M. Bragielの学位論文として提出され,学位審査・授与の対象となっている。
Rights
© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3071号
Diploma Number
甲口第414号
Granted Date
2017-03-23
Degree Name
Doctor of Philosophy
Grantor
Tokushima University
departments
Technical Support Department
Oral Sciences