ID | 109875 |
Title Transcription | シニクコウ シンシュツエキ チュウ ノ バイオマーカー オ モチイタ シシュウビョウ シンダン
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Title Alternative | Diagnosis of Periodontal Diseases using Biomarkers in Gingival Crevicular Fluid
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Author |
Kido, Jun-ichi
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Bando, Mika
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
Tokushima University Educator and Researcher Directory
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Inagaki, Yuji
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Ikuta, Takahisa
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Kajiura, Yukari
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Nakajima, Yukiko
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Sakamoto, Eijiro
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
Mihara, Chie
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Ninomiya, Masami
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Nishikawa, Yasufumi
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Lew, Jung-Hwan
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
Naruishi, Koji
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Nagata, Toshihiko
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
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Keywords | 歯周病診断
バイオマーカー
歯肉溝滲出液
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Content Type |
Journal Article
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Description | Periodontal diseases cause an inflammation and degradation of periodontal tissues and missing of teeth. The incidence rate of periodontal diseases is high in middle-aged and elderly people.
A reasonable diagnosis of periodontal diseases is very important to keep teeth, however, conventional examinations of periodontal diseases is not necessarily exact and objective. Gingival crevicular fluid (GCF) is an exudate secreted from periodontal tissues and contains many components including proteolytic enzymes, inflammatory cytokines, blood-associated proteins, cellular and bacterial fragments. Because some proteins in GCF are related to inflammation, tissue degradation and bone metabolism, those proteins have been studying as a diagnostic marker of periodontal diseases. GCF is noninvasively collected using a sterile paper strip and biomarkers are determined using enzyme-linked immunosorbent assay (ELISA) and enzyme activity assay. We identified calprotectin, an inflammationrelated protein, in GCF and calprotectin level in GCF from periodontitis sites was significantly higher than that of healthy control. Calprotectin level in GCF was positively correlated to gingival index and other biomarkers and decreased by periodontal treatments. Resistin is an adipocytokine and its level increases in some inflammatory diseases. Resistin level in GCF from periodontitis sites was high compared to the level of healthy control samples. Procollagen type I C-terminal peptide (PICP) is a biomarker for bone metabolism and its level was high in GCF collected from periodontitis sites. These results suggested that calprotectin, resistin and PICP are useful biomarkers for periodontal diseases. On the other hand, we showed that glycated albumin (GA), a marker of diabetes mellitus (DM), was contained in GCF and GA level in GCF from DM patients was significantly higher than that of non-DM individuals. Components in GCF may be biomarkers of systemic diseases as well as periodontal diseases and their determination will be useful diagnostic examination of some diseases. Recently, we have been studying the determining system of GCF calprotectin, including microchip ELISA, surface plasmon resonance assay and immuno-chromatography assay. When GCF biomarkers are determined using the determining systems, we will simply, exactly and objectively diagnose periodontal diseases at our dental offices. |
Journal Title |
Journal of Oral Health and Biosciences
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ISSN | 21887888
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NCID | AA12713630
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Publisher | 四国歯学会
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Volume | 29
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Issue | 1
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Start Page | 13
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End Page | 20
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Sort Key | 13
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Published Date | 2016-06-30
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EDB ID | |
FullText File | |
language |
jpn
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TextVersion |
Publisher
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departments |
Oral Sciences
University Hospital
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