ID | 80825 |
Author |
Kasai, Yuka
Department of Obstetrics and Gynecology, Tokushima Prefectural Central Hospital
Maegawa, Masahiko
Department of Obstetrics and Gynecology, Tokushima Prefectural Central Hospital
Yamamoto, Satoshi
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Kamada, Masaharu
Department of Obstetrics and Gynecology,Health Insurance Naruto Hospital
Yasui, Toshiyuki
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Uemura, Hirokazu
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
KAKEN Search Researchers
Kobayashi, Ayako
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Kaneyama, Masayo
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Tani, Anna
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Matsui, Sumika
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
KAKEN Search Researchers
Kuwahara, Akira
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
KAKEN Search Researchers
Matsuzaki, Toshiya
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
KAKEN Search Researchers
Furumoto, Hiroyuki
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Irahara, Minoru
Department of Obstetrics and Gynecology, Institute of Health Biosciences, the University of Tokushima Graduate School
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
|
Keywords | raloxifene
cytokine
whole blood assay
|
Content Type |
Journal Article
|
Description | Purpose : The aims of this study were to determine the effects of raloxifene
therapy on production of cytokines and in vitro effects of raloxifene on production of cytokines by whole blood cultures. Methods :We obtained samples of peripheral blood from 6 postmenopausal women with osteopenia at baseline and after 3 and 6 months of raloxifene therapy and 10 postmenopausal women who did not receive raloxifene therapy. Whole blood from raloxifene-treated women was stimulated with lipopolysaccharide (LPS) or phytohemeagglutinin (PHA). Whole blood from postmenopausal women who were not treated with raloxifene was preincubated with raloxifene at concentrations of 10-10-10-7 M and then stimulated with LPS or PHA. Concentrations of IL-1β, IL-4, IL-6, IL-12p40, IL-12p70, TNF-α and IFN-γ in the supernatant were measured by respective ELISAs. Results : In ex vivo cultures, raloxifene therapy inhibited LPS-stimulated production of IL-1β, IL-6, IL-12p40, IL-12p70 and TNF-α, but not PHA-stimulated production of IL-4 and IFN-γ. In in vitro cultures, raloxifene at a concentration (10-9 M) inhibited LPS-stimulated production of IL-1β, IL-6 and IL-12p40 and PHA-stimulated production of IFN-γ. Conclusions : Raloxifene therapy decreases the production of IL-1β, IL-6, IL-12 and TNF-α but not that of IL-4 and IFN-γ, suggesting that modulation of cytokines could play a role in the mechanisms of the osteoprotective effect of raloxifene. |
Journal Title |
The journal of medical investigation : JMI
|
ISSN | 13431420
|
NCID | AA11166929
|
Volume | 58
|
Issue | 1-2
|
Start Page | 110
|
End Page | 117
|
Sort Key | 110
|
Published Date | 2011-02
|
Remark | The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
|
EDB ID | |
FullText File | |
language |
eng
|
departments |
Medical Sciences
University Hospital
|