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ID 119198
Author
Kajimoto, Kazuaki National Institute of Advanced Industrial Science and Technology
Keywords
β-defensin 2
Cell-free protein synthesis system
Liposome
Drug delivery system
Porphyromonas gingivalis
Content Type
Journal Article
Description
β-defensin 2 (BD-2), an antimicrobial peptide (AMP), is expressed by oral epithelial cells and plays an important role in innate immunity of the oral cavity. Cell-free protein synthesis (CFPS) systems have been studied for the synthesis of various proteins, however, the synthesis of BD-2 by a CFPS system has not been extensively explored. Liposomes have been developed as tools for drug delivery. A delivery of liposome-encapsulated AMP to oral epithelium may be useful to prevent oral infectious diseases. In the present study, we investigated the antimicrobial activity of the BD-2 protein, artificially synthesized using a CFPS system and encapsulated in liposomes. BD-2 protein was artificially synthesized using template DNA and a reconstituted CFPS system and was identified by western blotting. Bilayer liposomes were prepared using 1,2-dioleoyl-sn-glycero-3-phospho-choline and 3-sn-phosphatidylcholine from egg yolk. The artificially synthesized BD-2 was encapsulated in liposomes, collected by ultrafiltration, and detected by western blotting. Human oral epithelial cells were cultured with the liposome-encapsulated BD-2 and the concentration of BD-2 in the cell lysate of the culture with the synthesized BD-2 was higher than that of the control cultures. The antimicrobial activity of the synthesized BD-2 was investigated by an adhesion assay of Porphyromonas gingivalis to oral epithelial cells. The artificially synthesized BD-2 and its liposome significantly inhibited adhesion of P. gingivalis to oral epithelial cells. These results suggest that artificially synthesized BD-2 and liposome-encapsulated BD-2 shows antimicrobial activity and can potentially play a role in oral healthcare for periodontal diseases.
Journal Title
Odontology
ISSN
16181255
16181247
NCID
AA11580928
Publisher
Springer Nature|The Society of The Nippon Dental University
Volume
111
Issue
4
Start Page
830
End Page
838
Published Date
2023-02-06
Remark
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use (https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms), but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s10266-023-00789-x
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DOI (Published Version)
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FullText File
language
eng
TextVersion
Author
departments
Institute of Advanced Medical Sciences
Oral Sciences
University Hospital