Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells

Ohigashi, Izumi; Tanaka, Yu; Kondo, Kenta; Fujimori, Sayumi; Kondo, Hiroyuki; Palin, Amy C.; Hoffmann, Victoria; Kozai, Mina; Matsushita, Yosuke; Uda, Shinsuke; Motosugi, Ryo; Hamazaki, Jun; Kubota, Hiroyuki; Murata, Shigeo; Tanaka, Keiji; Katagiri, Toyomasa; Kosako, Hidetaka; Takahama, Yousuke

doi:10.1016/j.celrep.2019.10.079

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Use this link to cite this item : https://repo.lib.tokushima-u.ac.jp/115049

ID	115049
Author	Ohigashi, Izumi University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers Tanaka, Yu NIH Kondo, Kenta NIH Fujimori, Sayumi University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers Kondo, Hiroyuki University of Tokushima Palin, Amy C. NIH Hoffmann, Victoria NIH Kozai, Mina University of Tokushima KAKEN Search Researchers Matsushita, Yosuke University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers Uda, Shinsuke Kyushu University Motosugi, Ryo The University of Tokyo Hamazaki, Jun The University of Tokyo Kubota, Hiroyuki Kyushu University Murata, Shigeo The University of Tokyo Tanaka, Keiji Tokyo Metropolitan Institute for Medical Science Katagiri, Toyomasa University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers Kosako, Hidetaka University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers Takahama, Yousuke NIH Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Content Type	Journal Article
Description	The thymic function to produce self-protective and self-tolerant T cells is chiefly mediated by cortical thymic epithelial cells (cTECs) and medullary TECs (mTECs). Recent studies including single-cell transcriptomic analyses have highlighted a rich diversity in functional mTEC subpopulations. Because of their limited cellularity, however, the biochemical characterization of TECs, including the proteomic profiling of cTECs and mTECs, has remained unestablished. Utilizing genetically modified mice that carry enlarged but functional thymuses, here we show a combination of proteomic and transcriptomic profiles for cTECs and mTECs, which identified signature molecules that characterize a developmental and functional contrast between cTECs and mTECs. Our results reveal a highly specific impact of the thymoproteasome on proteasome subunit composition in cTECs and provide an integrated trans-omics platform for further exploration of thymus biology.
Journal Title	Cell Reports
ISSN	22111247
Publisher	Elsevier
Volume	29
Issue	9
Start Page	2901
End Page	2916
Published Date	2019-11-26
Rights	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
EDB ID	361076
DOI (Published Version)	10.1016/j.celrep.2019.10.079
URL ( Publisher's Version )	https://doi.org/10.1016/j.celrep.2019.10.079
FullText File	celrep_29_9_2901.pdf 5.28 MB
language	eng
TextVersion	Publisher
departments	Institute of Advanced Medical Sciences