ID | 114556 |
Title Alternative | Metabolic remodeling of hyperactive rats
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Author |
Horiguchi, Taigo
Tokushima University
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Miyatake, Yumiko
Tokushima University|Wakayama Medical University
Miyoshi, Keiko
Tokushima University
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Hagita, Hiroko
Tokushima University
Sakaue, Hiroshi
Tokushima University
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Noma, Takafumi
Tokushima University
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Keywords | adenylate kinase
energy metabolism
microarray analysis
SPORTS rats
voluntary exercise
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Content Type |
Journal Article
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Description | Spontaneously Running Tokushima Shikoku (SPORTS) rat is a hyperactive rat strain. However, the causative mutation of this phenotype has not yet been identified. To investigate the molecular basis for the unique phenotype of SPORTS rats, we examined gene-expression profiles by microarray analyses. Among adenylate kinase isozymes that maintain the homeostasis of cellular adenine nucleotide composition in the cell, only adenylate kinase 1 is highly up-regulated in both exercised and sedentary SPORTS rats compared with wild-type (WT) rats, 5.5-fold and 3.3-fold, respectively. Further comparative analyses revealed that genes involved in glucose metabolism were up-regulated in skeletal muscle tissue of exercised SPORTS rats compared with sedentary mutants, whereas genes related to extracellular matrix or region were down-regulated compared with WT rats. In brain tissue of sedentary SPORTS rats, genes associated with defense and catecholamine metabolism were highly expressed compared with WT rats. These findings suggest that genetic mutation(s) in SPORTS rat remodels metabolic demands through differentially regulating gene expression regardless of exercise. Therefore, the SPORTS rats are useful animal model not only for further examining the effects of exercise on metabolism but also for deeply studying the molecular basis how mutation affect the psychological motivation with spontaneous voluntary exercise phenotype.
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Journal Title |
The Journal of Medical Investigation
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ISSN | 13496867
13431420
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NCID | AA12022913
AA11166929
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Publisher | Tokushima University Faculty of Medicine
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Volume | 67
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Issue | 1-2
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Start Page | 51
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End Page | 61
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Sort Key | 51
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Published Date | 2020-02
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
Publisher
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departments |
Oral Sciences
Medical Sciences
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