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ID 119161
Title Alternative
成長ホルモンシグナルにおけるα-klothoの関与
Author
Koike, Megumi Tokushima University
Sato, Tetsuhiko Nagoya Daini Red Cross Hospital
Komiya, Aoi Tokushima University
Miura, Mizuki Tokushima University
Higashi, Ayami Tokushima University
Ishikawa, Akane Tokushima University
Takayanagi, Kaori Tokushima University
Uga, Minori Tokushima University
Keywords
growth hormone
α-klotho
kidney
CKD
GH-resistant
Content Type
Thesis or Dissertation
Description
Growth hormone (GH) exerts multiple effects on different organs directly or via its main mediator, insulin-like growth factor1 (IGF1). In this study, we focused on the novel relationship between GH action and the antiaging hormone α-klotho. Immunofluorescent staining of α-klotho was observed in the renal distal tubules and pituitary glands of somatostatin- and GH-positive cells in wild-type (WT) mice. Treatment of 4-week-old WT mice with GH increased IGF1 mRNA expression in the pituitary gland, liver, heart, kidney, and bone but increased α-klotho mRNA expression only in the pituitary gland, kidney, and bone. Increased α-klotho protein levels were observed in the kidney but not in the pituitary gland. No induction of α-klotho RNA expression by GH was observed in juvenile mice with kidney disease, indicating GH resistance. Furthermore, GH and α-klotho supplementation in HEK293 cells transfected with GHR increased Janus kinase 2 mRNA (a GH downstream signal) expression compared to supple­mentation with GH alone. In conclusion, we suggest that 1) the kidney is the main source of secreted α-klotho, which is detected in blood by the downstream action of GH, 2) α-klotho induction by GH is resistant in kidney disease, and 3) α-klotho might be an enhanced regulator of GH signaling.
Journal Title
Journal of Clinical Biochemistry and Nutrition
ISSN
09120009
18805086
NCID
AA10710201
Publisher
Society for Free Radical Research Japan
Volume
74
Issue
3
Start Page
221
End Page
229
Published Date
2024
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Megumi Koikeの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3775号
Diploma Number
甲栄第303号
Granted Date
2024-02-22
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences