Total for the last 12 months
number of access : ?
number of downloads : ?
ID 118878
Title Alternative
脳梗塞モデルラットにおける虚血後の時期依存的な抗炎症性M2マクロファージ活性化変調の役割
Author
Kurashiki, Yoshitaka Tokushima University
Kinouchi, Tomoya Tokushima University
Sumiyoshi, Manabu Tokushima University
Kitazato, Keiko T Tokushima University
Keywords
Arginase-1
cerebral ischemia
interleukin-4
macrophage modulation
post-ischemic phase
Content Type
Thesis or Dissertation
Description
Cerebral ischemia triggers inflammatory changes, and early complications and unfavorable outcomes of endovascular thrombectomy for brain occlusion promote the recruitment of various cell types to the ischemic area. Although anti-inflammatory M2-type macrophages are thought to exert protective effects against cerebral ischemia, little has been clarified regarding the significance of post-ischemic phase-dependent modulation of M2-type macrophages. To test our hypothesis that post-ischemic phase-dependent modulation of macrophages represents a potential therapy against ischemic brain damage, the effects on rats of an M2-type macrophage-specific activator, Gc-protein macrophage-activating factor (GcMAF), were compared with vehicle-treated control rats in the acute (day 0–6) or subacute (day 7–13) phase after ischemia induction. Acute-phase GcMAF treatment augmented both anti-inflammatory CD163+M2-type- and pro-inflammatory CD16+ M1-type macrophages, resulting in no beneficial effects. Conversely, subacute-phase GcMAF injection increased only CD163+ M2-type macrophages accompanied by elevated mRNA levels of arginase-1 and interleukin-4. M2-type macrophages co-localized with CD36+ phagocytic cells led to clearance of the infarct area, which were abrogated by clodronate-liposomes. Expression of survival-related molecules on day 28 at the infarct border was augmented by GcMAF. These data provide new and important insights into the significance of M2-type macrophage-specific activation as post-ischemic phase-dependent therapy.
Journal Title
Journal of Cerebral Blood Flow & Metabolism
ISSN
15597016
0271678X
NCID
AA12510484
AA10458430
Publisher
International Society for Cerebral Blood Flow and Metabolism|Sage
Volume
43
Issue
4
Start Page
531
End Page
541
Published Date
2022-12-22
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Yoshitaka Kurashikiの学位論文として提出され,学位審査・授与の対象となっている。
Rights
Kurashiki Y, Kagusa H, Yagi K, et al. Role of post-ischemic phase-dependent modulation of anti-inflammatory M2-type macrophages against rat brain damage. Journal of Cerebral Blood Flow & Metabolism. 2023;43(4):531-541. Copyright © 2022 The Author(s). doi:10.1177/0271678X221147090
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3766号
Diploma Number
甲医第1591号
Granted Date
2023-12-28
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences
Bioscience and Bioindustry