カメザキ チヒロ Kyoto Pharmaceutical University
ナカシマ アミ Kyoto Pharmaceutical University
ヤマダ アサコ Kyoto Pharmaceutical University
ウエニシ サチコ Kyoto Pharmaceutical University
イシバシ ヒロシ Kyoto Pharmaceutical University
シブヤ ナツミ Department of Pharmaceutical Health Chemistry, Institute of Biomedical Sciences, Tokushima University, Graduate School
ハマ ススム Kyoto Pharmaceutical University
ホソイ シンゾウ Kyoto Pharmaceutical University
ヤマシタ エイジ AstaReal Co., Ltd.
Astaxanthin and vitamin E are both effective antioxidants that are frequently used in cosmetics, as food additives, and in to prevent oxidative damage. A combination of astaxanthin and vitamin E would be expected to show an additive anntioxidative effect. In this study, liposomes co-encapsulating astaxanthin and the vitamin E derivatives α-tocopherol (α-T) or tocotrienols (T3) were prepared, and the antioxidative activity of these liposomes toward singlet oxygen and hydroxyl radical was evaluated in vitro. Liposomes co-encapsulating astaxanthin and α-T showed no additive anntioxidative effect, while the actual scavenging activity of liposomes co-encapsulating astaxanthin and T3 was higher than the calculated additive activity. To clarify why this synergistic effect occurs, the most stable structure of astaxanthin in the presence of α-T or α-T3 was calculated. Only α-T3 was predicted to form hydrogen bonding with astaxanthin, and the astaxanthin polyene chain would partially interact with the α-T3 triene chain, which could explain why there was a synergistic effect between astaxanthin and T3 but not α-T. In conclusion, co-encapsulation of astaxanthin and T3 induces synergistic scavenging activity by intermolecular interactions between the two antioxidants.
Society for Free Radical Research Japan
Journal of Clinical Biochemistry and Nutrition
© 2016 Society for Free Radical Research Japan
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