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ID 116998
タイトル別表記
CRISPR-Cas9システムを用いた味覚受容体発現調節物質のスクリーニング系の開発
著者
別府, 香名 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Ansai, Satoshi Tohoku University
Ochiai, Nana Tokushima University
Terakawa, Mai Tokushima University
Mori, Marie Tokushima University
Tomoi, Takumi National Institute for Basic Biology
Sakamoto, Joe National Institute for Basic Biology
Kamei, Yasuhiro National Institute for Basic Biology|The Graduate University for Advanced Studies (SOKENDAI)
Naruse, Kiyoshi The Graduate University for Advanced Studies (SOKENDAI)|National Institute for Basic Biology
キーワード
taste 1 receptor 3
dysgeusia
medaka (Oryzias latipes)
CRISPR-Cas9 system
monosodium glutamate
5-fluorouracil
資料タイプ
学位論文
抄録
Taste recognition mediated by taste receptors is critical for the survival of animals in nature and is an important determinant of nutritional status and quality of life in humans. However, many factors including aging, diabetes, zinc deficiency, infection with influenza or cold viruses, and chemotherapy can trigger dysgeusia, for which a standard treatment has not been established. We here established an engineered strain of medaka (Oryzias latipes) that expresses green fluorescent protein (GFP) from the endogenous taste 1 receptor 3 (T1R3) gene locus with the use of the CRISPR-Cas9 system. This T1R3-GFP knock-in (KI) strain allows direct visualization of expression from this locus by monitoring of GFP fluorescence. The pattern of GFP expression in the T1R3-GFP KI fish thus mimicked that of endogenous T1R3 gene expression. Furthermore, exposure of T1R3-GFP KI medaka to water containing monosodium glutamate or the anticancer agent 5-fluorouracil resulted in an increase or decrease, respectively, in GFP fluorescence intensity, effects that also recapitulated those on T1R3 mRNA abundance. Finally, screening for agents that affect GFP fluorescence intensity in T1R3-GFP KI medaka identified tryptophan as an amino acid that increases T1R3 gene expression. The establishment of this screening system for taste receptor expression in medaka provides a new tool for the development of potential therapeutic agents for dysgeusia.
掲載誌名
Biochemical and Biophysical Research Communications
ISSN
0006291X
cat書誌ID
AA00564395
AA11542044
出版者
Elsevier
601
開始ページ
65
終了ページ
72
発行日
2022-02-23
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kana Beppuの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3600号
学位記番号
甲栄第288号
学位授与年月日
2022-03-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系