ID | 117004 |
タイトル別表記 | 紫外線発光ダイオードの照射は、そのピーク波長によって異なるウイルス分子を標的にすることで単純ヘルペスウイルスの感染性を減弱する
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著者 |
ブイ, ティ キム ガン
徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Fukushima, Shiho
Tokushima University
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キーワード | UV-LED
herpes simplex virus type 1
DNA photoproducs
viral lipids
Light-emitting diode
Ultraviolet
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資料タイプ |
学位論文
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抄録 | Herpes simplex virus type 1 (HSV-1) is an enveloped virus that mainly infects humans. Given its high global prevalence, disinfection is critical for reducing the risk of infection. Ultraviolet-light-emitting diodes (UV-LEDs) are eco-friendly irradiating modules with different peak wavelengths, but the molecules degraded by UV-LED irradiation have not been clarified. To identify the target viral molecules of UV-LEDs, we exposed HSV-1 suspensions to UV-LED irradiation at wavelengths of 260-, 280-, 310-, and 365-nm and measured viral DNA, protein, and lipid damage and infectivity in host cells. All UV-LEDs substantially reduced by inhibiting host cell transcription, but 260- and 280-nm UV-LEDs had significantly stronger virucidal efficiency than 310- and 365-nm UV-LEDs. Meanwhile, 260- and 280-nm UV-LEDs induced the formation of viral DNA photoproducts and the degradation of viral proteins and some phosphoglycerolipid species. Unlike 260- and 280-nm UV-LEDs, 310- and 365-nm UV-LEDs decreased the viral protein levels, but they did not drastically change the levels of viral DNA photoproducts and lipophilic metabolites. These results suggest that UV-LEDs reduce the infectivity of HSV-1 by targeting different viral molecules based on the peak wavelength. These findings could facilitate the optimization of UV-LED irradiation for viral inactivation.
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掲載誌名 |
Journal of Photochemistry and Photobiology B: Biology
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ISSN | 10111344
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cat書誌ID | AA10686173
AA11533983
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出版者 | Elsevier
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巻 | 228
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開始ページ | 112410
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発行日 | 2022-02-14
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備考 | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Thi Kim Ngan Buiの学位論文として提出され,学位審査・授与の対象となっている。 |
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第3602号
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学位記番号 | 甲栄第290号
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学位授与年月日 | 2022-03-23
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学位名 |
博士(栄養学)
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学位授与機関 |
徳島大学
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部局 |
医学系
理工学系
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