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ID 119259
タイトル別表記
Vibrio vulnificusから産生される細胞外小胞の特徴について
Characterization of OMVs by V. vulnificus
著者
東山, 諒 徳島大学大学院医科栄養学研究科(医科栄養学専攻)
Kanda, Yuna Tokushima University
下畑, 隆明 Tokushima University|Fukui Prefectural University KAKEN研究者をさがす
Fukushima, Shiho Tokushima University
Yamazaki, Kohei Kitasato University
Kashimoto, Takashige Kitasato University
キーワード
Infection
Outer Membrane Vesicle
Pathogenicity
Serine Protease
Vibrio Vulnificus
資料タイプ
学位論文
抄録
Vibrio vulnificus (V. vulnificus) is a halophilic gram-negative bacterium that inhabits coastal warm water and induce severe diseases such as primary septicemia. To investigate the mechanisms of rapid bacterial translocation on intestinal infection, we focused on outer membrane vesicles (OMVs), which are extracellular vesicles produced by Gram-negative bacteria and deliver virulence factors. However, there are very few studies on the pathogenicity or contents of V. vulnificus OMVs (Vv-OMVs). In this study, we investigated the effects of Vv-OMVs on host cells. Epithelial cells INT407 were stimulated with purified OMVs and morphological alterations and levels of lactate dehydrogenase (LDH) release were observed. In cells treated with OMVs, cell detachment without LDH release was observed, which exhibited different characteristics from cytotoxic cell detachment observed in V. vulnificus infection. Interestingly, OMVs from a Vibrio Vulnificus Hemolysin (VVH) and Multifunctional-autoprocessing repeats-in -toxin (MARTX) double-deletion mutant strain also caused cell detachment without LDH release. Our results suggested that the proteolytic function of a serine protease contained in Vv-OMVs may contribute to pathogenicity of V. vulnificus by assisting bacterial translocation. This study reveals a new pathogenic mechanism during V. vulnificus infections.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
出版者
Tokushima University Faculty of Medicine
71
1-2
開始ページ
102
終了ページ
112
並び順
102
発行日
2024-02
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Ryo Higashiyamaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3774号
学位記番号
甲栄第302号
学位授与年月日
2024-02-22
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系