ID | 113289 |
タイトル別表記 | COMBINATION OF IONS PROMOTES GINGIVAL FIBROBLAST MIGRATION
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著者 |
河原林, 啓太
Tokushima University
杉本, 明日菜
Tokushima University
宮嵜, 彩
Tokushima University
Kurogoushi, Rika
Tokushima University
Iwata, Kokoro
Tokushima University
Yamada, Aya
Tohoku University
Fukumoto, Satoshi
Tohoku University
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キーワード | gingival fibroblasts
cell migration
extracellular signal‑regulated kinase signaling
multiple‑ion solution
surface pre‑reacted glass‑ionomer filler
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資料タイプ |
学術雑誌論文
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抄録 | Wound healing is a dynamic process that involves highly coordinated cellular events, including proliferation and migration. Oral gingival fibroblasts serve a central role in maintaining oral mucosa homeostasis, and their functions include the coordination of physiological tissue repair. Recently, surface pre‑reacted glass‑ionomer (S‑PRG) fillers have been widely applied in the field of dental materials for the prevention of dental caries, due to an excellent ability to release fluoride (F). In addition to F, S‑PRG fillers are known to release several types of ions, including aluminum (Al), boron (B), sodium (Na), silicon (Si) and strontium (Sr). However, the influence of these ions on gingival fibroblasts remains unknown. The aim of the present study was to examine the effect of various concentrations of an S‑PRG filler eluate on the growth and migration of gingival fibroblasts. The human gingival fibroblast cell line HGF‑1 was treated with various dilutions of an eluent solution of S‑PRG, which contained 32.0 ppm Al, 1,488.6 ppm B, 505.0 ppm Na, 12.9 ppm Si, 156.5 ppm Sr and 136.5 ppm F. Treatment with eluate at a dilution of 1:10,000 was observed to significantly promote the migration of HGF‑1 cells. In addition, the current study evaluated the mechanism underlying the mediated cell migration by the S‑PRG solution and revealed that it activated the phosphorylation of extracellular signal‑regulated kinase 1/2 (ERK1/2), but not of p38. Furthermore, treatment with a MEK inhibitor blocked the cell migration induced by the solution. Taken together, these results suggest that S‑PRG fillers can stimulate HGF‑1 cell migration via the ERK1/2 signaling pathway, indicating that a dental material containing this type of filler is useful for oral mucosa homeostasis and wound healing.
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掲載誌名 |
Molecular Medicine Reports
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ISSN | 17912997
17913004
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出版者 | Spandidos Publications
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巻 | 19
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号 | 6
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開始ページ | 5039
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終了ページ | 5045
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発行日 | 2019-04-08
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
著者版
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部局 |
病院
歯学系
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