ID | 116451 |
タイトル別表記 | The role of AmeloD in tooth development
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著者 |
Chiba, Yuta
National Institutes of Health|Tohoku University
He, Bing
National Institutes of Health|Sichuan University
Yoshizaki, Keigo
National Institutes of Health|Kyushu University
Rhodes, Craig
National Institutes of Health
Ishijima, Muneaki
National Institutes of Health|Juntendo University
Bleck, Christopher K. E.
National Institutes of Health
Stempinski, Erin
National Institutes of Health
Chu, Emily Y.
National Institutes of Health
Nakamura, Takashi
National Institutes of Health|Tohoku University
de Vega, Susana
National Institutes of Health|Juntendo University
Saito, Kan
Tohoku University
Fukumoto, Satoshi
National Institutes of Health|Tohoku University
Yamada, Yoshihiko
National Institutes of Health
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資料タイプ |
学術雑誌論文
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抄録 | The development of ectodermal organs, such as teeth, requires epithelial–mesenchymal interactions. Basic helix–loop–helix (bHLH) transcription factors regulate various aspects of tissue development, and we have previously identified a bHLH transcription factor, AmeloD, from a tooth germ cDNA library. Here, we provide both in vitro and in vivo evidence that AmeloD is important in tooth development. We created AmeloD-knockout (KO) mice to identify the in vivo functions of AmeloD that are critical for tooth morphogenesis. We found that AmeloD-KO mice developed enamel hypoplasia and small teeth because of increased expression of E-cadherin in inner enamel epithelial (IEE) cells, and it may cause inhibition of the cell migration. We used the CLDE dental epithelial cell line to conduct further mechanistic analyses to determine whether AmeloD overexpression in CLDE cells suppresses E-cadherin expression and promotes cell migration. Knockout of epiprofin (Epfn), another transcription factor required for tooth morphogenesis and development, and analysis of AmeloD expression and deletion revealed that AmeloD also contributed to multiple tooth formation in Epfn-KO mice by promoting the invasion of dental epithelial cells into the mesenchymal region. Thus, AmeloD appears to play an important role in tooth morphogenesis by modulating E-cadherin and dental epithelial–mesenchymal interactions. These findings provide detailed insights into the mechanism of ectodermal organ development.
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掲載誌名 |
Journal of Biological Chemistry
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ISSN | 00219258
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cat書誌ID | AA1202441X
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出版者 | American Society for Biochemistry and Molecular Biology|Elsevier
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巻 | 294
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号 | 10
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開始ページ | 3406
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終了ページ | 3418
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発行日 | 2018-11-30
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権利情報 | This is an Open Access article under the CC BY license(https://creativecommons.org/licenses/by/4.0/).
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言語 |
eng
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著者版フラグ |
出版社版
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部局 |
歯学系
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