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偏光分解顕微鏡を用いたアルコール性肝線維症の線維化の量的・質的評価系の開発
著者
松崎, 紗弥 徳島大学大学院医学研究科(医学専攻)
髙成, 広起 Tokushima University|Nihon University 徳島大学 教育研究者総覧
Hayashi, Yuri Tokushima University
Hayashi, Yusaku Tokushima University
Oshikata, Haruto Tokushima University
Kimura, Satoko Tokyo Medical Examiner’s Office
キーワード
SHGimage
hepatic scleosis
collagen fiber
fatty liver disease
資料タイプ
学位論文
抄録
Liver fibrosis is assessed mainly by conventional staining or second harmonic generation (SHG) microscopy, which can only provide collagen content in fibrotic area. We propose to use polarization-resolved SHG (PR-SHG) microscopy to quantify liver fibrosis in terms of collagen fiber orientation and crystallization. Liver samples obtained from autopsy cases with fibrosis stage of F0–F4 were evaluated with an SHG microscope, and 12 consecutive PR-SHG images were acquired while changing the polarization azimuth angle of the irradiated laser from 0° to 165° in 15° increments using polarizer. The fiber orientation angle (φ) and degree (ρ) of collagen were estimated from the images. The SHG-positive area increased as the fibrosis stage progressed, which was well consistent with Sirius Red staining. The value of φ was random regardless of fibrosis stage. The mean value of ρ (ρ-mean), which represents collagen fiber crystallinity, varied more as fibrosis progressed to stage F3, and converged to a significantly higher value in F4 than in other stages. Spatial dispersion of ρ (ρ-entropy) also showed increased variation in the stage F3 and decreased variation in the stage F4. It was shown that PR-SHG could provide new information on the properties of collagen fibers in human liver fibrosis.
掲載誌名
Scientific Reports
ISSN
20452322
出版者
Springer Nature
13
開始ページ
22100
発行日
2023-12-13
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Saya Matsuzakiの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3785号
学位記番号
甲医第1600号
学位授与年月日
2024-03-07
学位名
博士(医学)
学位授与機関
徳島大学
部局
ポストLEDフォトニクス研究所
病院
理工学系
医学系