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ID 117908
著者
Husseini, Rabab A. Zagazig University|Tokushima University
福田, 達也 Wakayama Medical University KAKEN研究者をさがす
Hasan, Azza A. Zagazig University
El Megrab, Nagia A. Zagazig University
キーワード
prophylactic cancer vaccine
iontophoresis
polyplex nanoparticle
antigen peptide
cytosine-phosphate- guanosine motif (CpG-ODN) adjuvant
melanoma
資料タイプ
学術雑誌論文
抄録
Although the strategy in cancer vaccination is to provide a therapeutic effect against an established tumor, there is an urgent need to develop prophylactic vaccines for non-viral cancers. In this study, we prepared polyplex nanoparticles through electrostatic interactions between a positively-charged modified tumor associated antigen, namely human derived melanoma gp10025–33 peptide (KVPRNQDWL-RRRR), and a negatively charged cytosine-phosphate-guanosine motif (CpG-ODN) adjuvant. We previously demonstrated successful transdermal delivery of various hydrophilic macromolecules by iontophoresis (IP) using weak electricity. Herein, we investigated the effectiveness of IP in the transdermal delivery of a prophylactic polyplex vaccine. IP was successful in establishing a homogenous distribution of the vaccine throughout skin. Efficacy of the vaccine was demonstrated against melanoma growth. A significant tumor regression effect was observed, which was confirmed by elevated mRNA expression levels of various cytokines, mainly interferon (IFN)-γ, as well as infiltration of cytotoxic CD8+ T cells. Additionally, we evaluated the therapeutic effect of the vaccine and we found a significant reduction in tumor burden. Stimulation of systemic immunity was confirmed by upregulation of IFN-γ. This is the first report to demonstrate the use of IP in the transdermal delivery of a prophylactic melanoma vaccine.
掲載誌名
Biological and Pharmaceutical Bulletin
ISSN
13475215
cat書誌ID
AA11696048
出版者
The Pharmaceutical Society of Japan
46
3
開始ページ
494
終了ページ
504
発行日
2023-03-01
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
薬学系