ID | 110137 |
タイトル別表記 | ヒト腱・靭帯のプロテオーム解析 : 結合組織における不溶性細胞外マトリックスの可溶化と解析
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著者 |
合田, 有一郎
Tokushima University
小坂, 浩史
Tokushima University
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キーワード | 不溶性細胞外マトリックス
コラーゲン
エラスチン
プロテオーム解析
yellow ligament
Achilles tendon
elastin
collagen
proteome
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資料タイプ |
学位論文
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抄録 | Connective tissues such as tendon, ligament and cartilage are mostly composed of extracellular matrix (ECM). These tissues are insoluble, mainly due to the highly cross-linked ECM proteins such as collagens. Difficulties obtaining suitable samples for mass spectrometric analysis render the application of modern proteomic technologies difficult. Complete solubilization of them would not only elucidate protein composition of normal tissues but also reveal pathophysiology of pathological tissues. Here we report complete solubilization of human Achilles tendon and yellow ligament, which is achieved by chemical digestion combined with successive protease treatment including elastase. The digestion mixture was subjected to liquid chromatography-mass spectrometry. The low specificity of elastase was overcome by accurate mass analysis achieved using FT-ICR-MS. In addition to the detailed proteome of both tissues, we also quantitatively determine the major protein composition of samples, by measuring peak area of some characteristic peptides detected in tissue samples and in purified proteins. As a result, differences between human Achilles tendon and yellow ligament were elucidated at molecular level.
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掲載誌名 |
Journal of Proteome Research
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ISSN | 15353893
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cat書誌ID | AA11638846
AA12442770
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出版者 | ACS Publications
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巻 | 15
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号 | 12
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開始ページ | 4709
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終了ページ | 4721
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発行日 | 2016-10-17
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備考 | 内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201705191001.pdf 論文本文:k3009_fulltext.pdf 著者の申請により要約(2017-05-19公開)に替えて論文全文を公開(2018-06-14) 本論文は, 著者Nori Satoの学位論文として提出され, 学位審査・授与の対象となっている。 |
権利情報 | Copyright © 2016 American Chemical Society
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第3009号
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学位記番号 | 甲医第1313号
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学位授与年月日 | 2017-02-23
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学位名 |
博士(医学)
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学位授与機関 |
徳島大学
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部局 |
病院
先端酵素学研究所
医学系
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