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ID 118290
タイトル別表記
Vibrio vulnificus創傷感染モデルマウスにおける骨格筋内の特徴的な代謝変化
Metabolic Changing in V. vulnificus-Infected Tissue
著者
石田, 快 徳島大学大学院医科栄養学研究科(医科栄養学専攻) 徳島大学 教育研究者総覧
下畑, 隆明 Tokushima University|Fukui Prefectural University KAKEN研究者をさがす
Kanda, Yuna Tokushima University
Nguyen, Anh Quoc National Institute of Nutrition
Masuda, Rumiko Tokushima University
Yamazaki, Kohei Kitasato University
Kashimoto, Takashige Kitasato University
キーワード
Vibrio vulnificus
CE-TOFMS
host metabolomics
TCA cycle
MARTX toxin
資料タイプ
学位論文
抄録
Vibrio vulnificus is a bacterium that inhabits warm seawater or brackish water environments and causes foodborne diseases and wound infections. In severe cases, V. vulnificus invades the skeletal muscle tissue, where bacterial proliferation leads to septicemia and necrotizing fasciitis with high mortality. Despite this characteristic, information on metabolic changes in tissue infected with V. vulnificus is not available. Here, we elucidated the metabolic changes in V. vulnificus-infected mouse skeletal muscle using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). Metabolome analysis revealed changes in muscle catabolites and energy metabolites during V. vulnificus infection. In particular, succinic acid accumulated but fumaric acid decreased in the infected muscle. However, the virulence factor deletion mutant revealed that changes in metabolites and bacterial proliferation were abolished in skeletal muscle infected with a multifunctional-autoprocessing repeats-in-toxin (MARTX) mutant. On the other hand, mice that were immunosuppressed via cyclophosphamide (CPA) treatment exhibited a similar level of bacterial counts and metabolites between the wild type and MARTX mutant. Therefore, our data indicate that V. vulnificus induces metabolic changes in mouse skeletal muscle and proliferates by using the MARTX toxin to evade the host immune system. This study indicates a new correlation between V. vulnificus infections and metabolic changes that lead to severe reactions or damage to host skeletal muscle.
掲載誌名
mSystems
ISSN
23795077
出版者
American Society for Microbiology
8
2
開始ページ
e00682-22
発行日
2023-03-20
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kai Ishidaの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2023 Ishida et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. (https://creativecommons.org/licenses/by/4.0/)
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3734号
学位記番号
甲栄第299号
学位授与年月日
2023-03-24
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系