ID | 114755 |
タイトル別表記 | Epigenetic modulation on sphere
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著者 | |
キーワード | Cancer stem cell
Sphere
Epigenetic modulation
Valproic acid
DNA methylation
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資料タイプ |
学術雑誌論文
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抄録 | Background : Cancer stem cell properties are highly relevant to the biology of treatment-resistant cancers. Epigenetic modification regulates gene expressions by chromatin remodeling during malignant transformation. The aim of this study was to elucidate the possible strategy for cancer stem cells focusing on epigenetic modification. Methods : We made cancer sphere from HepG2 cells, and we added Histone deacetylase (HDAC) inhibitor, valproic acid to cancer sphere. And we compared methylation status and the gene expression between normal HepG2 and cancer sphere groups, and between cancer sphere and sphere with HDAC inhibitor treatment groups. Results : Valproic acid (VPA) cancelled this spheroid formation. In comparison between normal HepG2 and cancer sphere, the number of methylation status changes more than 0.1 of beta level was 826 probes, and we could isolate some epithelial-mesenchymal transition (EMT) related genes. And VPA reduced the expressions of EMT related genes in sphere with RT-PCR. On the other hand, in comparison between cancer sphere and sphere with VPA treatment, we detected 29 probe of methylation status change, and VPA reduced the expressions of Bcl-6 in sphere. Conclusions : HDAC inhibitor affected the methylation status of cancer stem cells. Histone-acetylation might overcome treatmet-resistant cancer through the regulation of cancer stem cell.
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掲載誌名 |
The Journal of Medical Investigation
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ISSN | 13496867
13431420
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cat書誌ID | AA12022913
AA11166929
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出版者 | Tokushima University Faculty of Medicine
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巻 | 67
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号 | 1-2
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開始ページ | 70
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終了ページ | 74
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並び順 | 70
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発行日 | 2020-02
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備考 | 著者英表記誤記あり (誤)Shinihiro Yamada→(正)Shinichiro Yamada
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EDB ID | |
出版社版DOI | |
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フルテキストファイル | |
言語 |
eng
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著者版フラグ |
出版社版
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部局 |
病院
医学系
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