The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway

Purpose: Epigallocatechin 3-gallate (EGCG), a green tea polyphenol, has been shown to have anti-oxidant and anti-inflammatory effects in vitro and in vivo. The aim of this study was to investigate the effects and mechanism of EGCG on isolated pancreatic islets as pre-conditioning for pancreatic islet transplantation.
Methods: The pancreatic islets were divided into two groups: an islet culture medium group (control) and an islet culture medium with EGCG (100 μM) group. We investigated the islet viability, Nrf2 expression, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) mRNA. Five hundred islet equivalents after 12 h of culture for the EGCG 100 μM and control group were transplanted under the kidney capsule of streptozotocin (STZ)-induced diabetic ICR mice.
Results: The cell viability and insulin secretion ability in the EGCG group were preserved, and the nuclear translocation of Nrf2 was increased in the EGCG group (p<0.01). While the HO-1 mRNA levels were also higher in the EGCG group than in the control group (p<0.05), the ROS production was lower (p<0.01). An in vivo functional assessment showed that the blood glucose level had decreased in the EGCG group after transplantation (p<0.01).
Conclusion: EGCG protects the viability and function of islets by suppressing ROS production via the Nrf2 pathway.

内容要旨・審査要旨・論文本文の公開
本論文は，著者Atsushi Takataの学位論文として提出され，学位審査・授与の対象となっている。
This is a post-peer-review, pre-copyedit version of an article published in Surgery Today. The final authenticated version is available online at: https://doi.org/10.1007/s00595-019-1761-0