ID | 112365 |
著者 |
Kono, Hitomi
Okayama University
Habuta, Munenori
Okayama University
Bando, Tetsuya
Okayama University
Sato, Keita
Okayama University
Inoue, Junji
Okayama University
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資料タイプ |
学術雑誌論文
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抄録 | The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is a rapid gene-targeting technology that does not require embryonic stem cells. To demonstrate dosage effects of the Pax6 gene on eye formation, we generated Pax6-deficient mice with the CRISPR/Cas system. Eyes of founder embryos at embryonic day (E) 16.5 were examined and categorized according to macroscopic phenotype as class 1 (small eye with distinct pigmentation), class 2 (pigmentation without eye globes), or class 3 (no pigmentation and no eyes). Histologically, class 1 eyes were abnormally small in size with lens still attached to the cornea at E16.5. Class 2 eyes had no lens and distorted convoluted retinas. Class 3 eyes had only rudimentary optic vesicle-like tissues or histological anophthalmia. Genotyping of neck tissue cells from the founder embryos revealed somatic mosaicism and allelic complexity for Pax6. Relationships between eye phenotype and genotype were developed. The present results demonstrated that development of the lens from the surface ectoderm requires a higher gene dose of Pax6 than development of the retina from the optic vesicle. We further anticipate that mice with somatic mosaicism in a targeted gene generated by CRISPR/Cas-mediated genome editing will give some insights for understanding the complexity in human congenital diseases that occur in mosaic form.
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掲載誌名 |
Scientific Reports
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ISSN | 20452322
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出版者 | Springer Nature
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巻 | 7
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開始ページ | 53
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発行日 | 2017-03-03
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備考 | Supplementary Information 1 : srep_7_53_s1.pdf
Supplementary Information 2 : srep_7_53_s2.pdf |
権利情報 | © The Author(s) 2017
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
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言語 |
eng
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部局 |
病院
先端酵素学研究所
生物資源系
歯学系
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