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ID 117211
著者
Ishimaru, Soichiro Tokushima University
Wai, Christine (Yee-Yan) The Chinese University of Hong Kong
Endo, Aiko Tokushima University
Kojima, Takumi Tokushima University
小林, 智子 Tokushima University
Sumida, Satoshi Tokushima University
Kakimoto, Takumi Tokushima University
Miyakami, Yuko Tokushima University
キーワード
food allergy
epicutaneous sensitization
shellfish
shrimp
tropomyosin
skin
Raman microscopy
tape-strip
資料タイプ
学術雑誌論文
抄録
Background: Shellfish allergy is one of the most common food allergies. Recent studies have shown that sensitization to allergens via the skin is involved in the development of food allergies. In this study, a mouse model of shrimp allergy was generated by epicutaneous sensitization and used to identify skin conditions associated with susceptibility to sensitization. Methods: Four-week-old female BALB/c mice were sensitized by repeated application of 0.1 mg of tropomyosin to tape-stripped skin on days 0, 7, and 15, followed by a challenge on days 28 and 35. Results: Epicutaneously sensitized mice exhibited higher serum levels of tropomyosin-specific IgE on day 15 than control mice. After the oral challenge, model mice had higher anaphylaxis scores and lower rectal temperature. After three tape-strip treatments for sensitization, the skin was analyzed by Raman microscopy. The sensitized mice exhibited lower relative intensities of Raman bands at 399, 915, and 1073 cm−1 than control mice, which could be helpful noninvasive markers in screening for potential sensitization via the skin. Conclusions: An epicutaneous sensitization shellfish allergy model was generated. This model will be useful in studies to elucidate the pathogenesis of skin sensitization. Raman microscopy may also be valuable for capturing subtle skin changes leading to sensitization.
掲載誌名
Applied Sciences
ISSN
20763417
出版者
MDPI
12
7
開始ページ
3566
発行日
2022-03-31
権利情報
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系
理工学系
病院