ID | 117211 |
著者 |
Ishimaru, Soichiro
Tokushima University
Wai, Christine (Yee-Yan)
The Chinese University of Hong Kong
Endo, Aiko
Tokushima University
Kojima, Takumi
Tokushima University
小林, 智子
Tokushima University
Sumida, Satoshi
Tokushima University
Kakimoto, Takumi
Tokushima University
Miyakami, Yuko
Tokushima University
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キーワード | food allergy
epicutaneous sensitization
shellfish
shrimp
tropomyosin
skin
Raman microscopy
tape-strip
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資料タイプ |
学術雑誌論文
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抄録 | Background: Shellfish allergy is one of the most common food allergies. Recent studies have shown that sensitization to allergens via the skin is involved in the development of food allergies. In this study, a mouse model of shrimp allergy was generated by epicutaneous sensitization and used to identify skin conditions associated with susceptibility to sensitization. Methods: Four-week-old female BALB/c mice were sensitized by repeated application of 0.1 mg of tropomyosin to tape-stripped skin on days 0, 7, and 15, followed by a challenge on days 28 and 35. Results: Epicutaneously sensitized mice exhibited higher serum levels of tropomyosin-specific IgE on day 15 than control mice. After the oral challenge, model mice had higher anaphylaxis scores and lower rectal temperature. After three tape-strip treatments for sensitization, the skin was analyzed by Raman microscopy. The sensitized mice exhibited lower relative intensities of Raman bands at 399, 915, and 1073 cm−1 than control mice, which could be helpful noninvasive markers in screening for potential sensitization via the skin. Conclusions: An epicutaneous sensitization shellfish allergy model was generated. This model will be useful in studies to elucidate the pathogenesis of skin sensitization. Raman microscopy may also be valuable for capturing subtle skin changes leading to sensitization.
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掲載誌名 |
Applied Sciences
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ISSN | 20763417
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出版者 | MDPI
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巻 | 12
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号 | 7
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開始ページ | 3566
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発行日 | 2022-03-31
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権利情報 | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
出版社版
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部局 |
医学系
理工学系
病院
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