ID | 114734 |
タイトル別表記 | 単球由来線維細胞のパラクライン効果を介した線維化促進作用の機能解析
Fibrocytes activate fibroblasts by PDGF.
|
著者 |
阿部, 秀一
徳島大学大学院医科学教育部(医学専攻)
Kishi, Masami
Tokushima University
Takahashi, Naoki
Tokushima University
|
キーワード | fibrocyte
fibroblast
idiopathic pulmonary fibrosis
PDGF
|
資料タイプ |
学位論文
|
抄録 | Fibrocytes, which are bone marrow-derived collagen-producing cells, were reported to play a role in the pathogenesis of pulmonary fibrosis. However, their function in pulmonary fibrosis is unclear. We analyzed their function compared with that of monocytes and localization in fibrotic tissues in patients with idiopathic pulmonary fibrosis (IPF). We compared the gene expression profile of monocyte-derived fibrocytes with that of monocytes by microarray analysis. Proliferation and differentiation into myofibroblasts were examined by 3H-thymidine incorporation assay and Western blotting. We measured the level of growth factors in the culture supernatant of fibrocytes by ELISA. The localization of fibrocytes in lung tissues of patients with IPF was determined by immunofluorescence staining. Compared with monocytes, fibrocytes had higher expression of extracellular matrix- and growth factor-encoding genes, including PDGF-B, FGF-2 and VEGF-B. Although fibrocytes did not proliferate in response to PDGF, co-culture of fibrocytes stimulated the growth of lung fibroblasts through the production of PDGF-BB. In the lung of IPF patients, CD45+Collagen-I+FSP-1+ cells, which have a similar phenotype to fibrocytes, were detected and co-stained with anti-PDGF antibody. This study suggested that fibrocytes function in pulmonary fibrosis partly by producing PDGF in the lungs of IPF patients.
|
掲載誌名 |
The Journal of Medical Investigation
|
ISSN | 13496867
13431420
|
cat書誌ID | AA12022913
AA11166929
|
出版者 | Tokushima University Faculty of Medicine
|
巻 | 67
|
号 | 1-2
|
開始ページ | 102
|
終了ページ | 112
|
並び順 | 102
|
発行日 | 2020-02
|
備考 | 内容要旨・審査要旨・論文本文の公開
本論文は, 著者Shuichi Abeの学位論文として提出され, 学位審査・授与の対象となっている。 |
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
|
著者版フラグ |
博士論文全文を含む
|
文科省報告番号 | 甲第3363号
|
学位記番号 | 甲医第1446号
|
学位授与年月日 | 2020-03-05
|
学位名 |
博士(医学)
|
学位授与機関 |
徳島大学
|
部局 |
病院
医学系
|