MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells

Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3'-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation in osteoblasts by up-regulating miR-494-3p followed by FGFR2 and ROCK1 gene repressions.

内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201507171004.pdf
審査要旨 : LID201507171005.pdf
論文本文 : k2858_fulltext.pdf
本論文は，著者Yuki Iwawakiの学位論文として提出され，学位審査・授与の対象となっている。
著者の申請により要約(2015-07-18公開)に替えて論文全文を公開(2019-08-22)

Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
This manuscript version is made available under the CC-BY-NC-ND 4.0 license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )