ID | 115728 |
著者 |
Yamamura, Shohei
National Institute of Advanced Industrial Science and Technology
Yatsushiro, Shouki
National Institute of Advanced Industrial Science and Technology
Yamaguchi, Yuka
National Institute of Advanced Industrial Science and Technology
Kataoka, Masatoshi
National Institute of Advanced Industrial Science and Technology
|
キーワード | microRNA
RNase protection assay
microchip electrophoresis
|
資料タイプ |
学術雑誌論文
|
抄録 | The analysis of a microRNA (miRNA), miR-222 isolated from the PC12 cell line, was performed by use of the ribonuclease (RNase) protection assay, cyanine 5 (Cy5)-labeled miR-222 riboprobe, and a Hitachi SV1210 microchip electrophoresis system, which can be used to evaluate the integrity of total RNA. The fluorescence intensity corresponding to the protected RNA fragment increased in a dose-dependent manner with respect to the complementary-strand RNA. More highly sensitive detection of miRNA by microchip electrophoresis than by conventional method using fluorescence-labeled riboprobe could be obtained in 180 s. An obvious increase in miR-222 expression induced by nerve growth factor in PC12 cells could be observed. These results clearly indicate the potential of microchip electrophoresis for the analysis of miRNA using RNase protection assay with a fluorescence-labeled riboprobe.
|
掲載誌名 |
Sensors
|
ISSN | 14248220
|
出版者 | MDPI
|
巻 | 12
|
号 | 6
|
開始ページ | 7576
|
終了ページ | 7586
|
発行日 | 2012-05-31
|
権利情報 | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
|
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
|
著者版フラグ |
出版社版
|
部局 |
病院
先端酵素学研究所
|