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ID 115728
著者
Yamamura, Shohei National Institute of Advanced Industrial Science and Technology
Yatsushiro, Shouki National Institute of Advanced Industrial Science and Technology
Yamaguchi, Yuka National Institute of Advanced Industrial Science and Technology
阿部, 佳織 National Institute of Advanced Industrial Science and Technology KAKEN研究者をさがす
Kataoka, Masatoshi National Institute of Advanced Industrial Science and Technology
キーワード
microRNA
RNase protection assay
microchip electrophoresis
資料タイプ
学術雑誌論文
抄録
The analysis of a microRNA (miRNA), miR-222 isolated from the PC12 cell line, was performed by use of the ribonuclease (RNase) protection assay, cyanine 5 (Cy5)-labeled miR-222 riboprobe, and a Hitachi SV1210 microchip electrophoresis system, which can be used to evaluate the integrity of total RNA. The fluorescence intensity corresponding to the protected RNA fragment increased in a dose-dependent manner with respect to the complementary-strand RNA. More highly sensitive detection of miRNA by microchip electrophoresis than by conventional method using fluorescence-labeled riboprobe could be obtained in 180 s. An obvious increase in miR-222 expression induced by nerve growth factor in PC12 cells could be observed. These results clearly indicate the potential of microchip electrophoresis for the analysis of miRNA using RNase protection assay with a fluorescence-labeled riboprobe.
掲載誌名
Sensors
ISSN
14248220
出版者
MDPI
12
6
開始ページ
7576
終了ページ
7586
発行日
2012-05-31
権利情報
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
病院
先端酵素学研究所