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ID 118052
タイトル別表記
ホメオボックス型転写因子Irx3はWnt5aの発現に関与し象牙芽細胞の増殖と分化に調整する
著者
ナルウィディナ, アンリザンディ- 徳島大学大学院口腔科学研究科(口腔科学専攻)
宮嵜, 彩 Tokushima University
Iwata, Kokoro Tokyo Medical and Dental University
Kurogoushi, Rika Tokyo Medical and Dental University
杉本, 明日菜 Tokyo Medical and Dental University
河原林, 啓太 Tokushima University
Yamakawa, Yoshihito Tokushima University
Yoshizaki, Keigo Kyushu University
Fukumoto, Satoshi Kyushu University
岩本, 勉 Tokyo Medical and Dental University KAKEN研究者をさがす
キーワード
Iroquois homeobox genes
Irx3
Odontoblasts
Cell proliferation
Cell differentiation
Wnt5a
資料タイプ
学位論文
抄録
Iroquois homeobox (Irx) genes are TALE-class homeobox genes that are evolutionarily conserved across species and have multiple critical cellular functions in fundamental tissue development processes. Previous studies have shown that Irxs genes are expressed during tooth development. However, the precise roles of genes in teeth remain unclear. Here, we demonstrated for the first time that Irx3 is an essential molecule for the proliferation and differentiation of odontoblasts. Using cDNA synthesized from postnatal day 1 (P1) tooth germs, we examined the expression of all Irx genes (Irx1-Irx6) by RT-PCR and found that all genes except Irx4 were expressed in the tooth tissue. Irx1-Irx3 a were expressed in the dental epithelial cell line M3H1 cells, while Irx3 and Irx5 were expressed in the dental mesenchymal cell line mDP cells. Only Irx3 was expressed in both undifferentiated cell lines. Immunostaining also revealed the presence of IRX3 in the dental epithelial cells and mesenchymal condensation. Inhibition of endogenous Irx3 by siRNA blocks the proliferation and differentiation of mDP cells. Wnt3a, Wnt5a, and Bmp4 are factors involved in odontoblast differentiation and were highly expressed in mDP cells by quantitative PCR analysis. Interestingly, the expression of Wnt5a (but not Wnt3a or Bmp4) was suppressed by Irx3 siRNA. These results suggest that Irx3 plays an essential role in part through the regulation of Wnt5a expression during odontoblast proliferation and differentiation.
掲載誌名
Biochemical and Biophysical Research Communications
ISSN
0006291X
10902104
cat書誌ID
AA00564395
AA11542044
出版者
Elsevier
650
開始ページ
47
終了ページ
54
発行日
2023-02-04
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Anrizandy Narwidinaの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3748号
学位記番号
甲口第498号
学位授与年月日
2023-09-20
学位名
博士(歯学)
学位授与機関
徳島大学
部局
病院
歯学系