ID | 119161 |
タイトル別表記 | 成長ホルモンシグナルにおけるα-klothoの関与
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著者 |
小池, 萌
徳島大学大学院医科栄養学研究科(医科栄養学専攻)
Sato, Tetsuhiko
Nagoya Daini Red Cross Hospital
Komiya, Aoi
Tokushima University
Miura, Mizuki
Tokushima University
Higashi, Ayami
Tokushima University
Ishikawa, Akane
Tokushima University
Takayanagi, Kaori
Tokushima University
Uga, Minori
Tokushima University
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キーワード | growth hormone
α-klotho
kidney
CKD
GH-resistant
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資料タイプ |
学位論文
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抄録 | Growth hormone (GH) exerts multiple effects on different organs directly or via its main mediator, insulin-like growth factor1 (IGF1). In this study, we focused on the novel relationship between GH action and the antiaging hormone α-klotho. Immunofluorescent staining of α-klotho was observed in the renal distal tubules and pituitary glands of somatostatin- and GH-positive cells in wild-type (WT) mice. Treatment of 4-week-old WT mice with GH increased IGF1 mRNA expression in the pituitary gland, liver, heart, kidney, and bone but increased α-klotho mRNA expression only in the pituitary gland, kidney, and bone. Increased α-klotho protein levels were observed in the kidney but not in the pituitary gland. No induction of α-klotho RNA expression by GH was observed in juvenile mice with kidney disease, indicating GH resistance. Furthermore, GH and α-klotho supplementation in HEK293 cells transfected with GHR increased Janus kinase 2 mRNA (a GH downstream signal) expression compared to supplementation with GH alone. In conclusion, we suggest that 1) the kidney is the main source of secreted α-klotho, which is detected in blood by the downstream action of GH, 2) α-klotho induction by GH is resistant in kidney disease, and 3) α-klotho might be an enhanced regulator of GH signaling.
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掲載誌名 |
Journal of Clinical Biochemistry and Nutrition
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ISSN | 09120009
18805086
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cat書誌ID | AA10710201
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出版者 | Society for Free Radical Research Japan
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巻 | 74
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号 | 3
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開始ページ | 221
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終了ページ | 229
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発行日 | 2024
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備考 | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Megumi Koikeの学位論文として提出され,学位審査・授与の対象となっている。 |
権利情報 | This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第3775号
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学位記番号 | 甲栄第303号
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学位授与年月日 | 2024-02-22
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学位名 |
博士(栄養学)
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学位授与機関 |
徳島大学
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部局 |
医学系
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