ID | 116060 |
タイトル別表記 | 変形性顎関節症の進展におけるp21の免疫調節作用
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著者 |
フゥレル オチル, ツェンドスレン
徳島大学大学院口腔科学教育部(口腔科学専攻)
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キーワード | p21
chondrocytes
temporomandibular joint
osteoarthritis
metalloprotease
chondrocyte
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資料タイプ |
学位論文
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抄録 | Objective: We aimed to identify the immunoregulatory role of the cyclin-dependent kinase inhibitor p21 in the homeostasis of mandibular condylar cartilage affected by mechanical stress.
Materials and Methods: Ten C57BL/6 wild-type (WT) and ten p21–/– mice aged 8 weeks were divided into the untreated and treated groups. In the treated groups, mechanical stress was applied to the temporomandibular joint (TMJ) through forced mouth opening for 3 h/day for 7 days. The dissected TMJs were assessed using micro-CT, histology, and immunohistochemistry. Results: Treated p21–/– condyles with mechanical stress revealed more severe subchondral bone destruction, with thinner cartilage layers and smaller proteoglycan area relative to treated WT condyles; untreated WT and p21–/– condyles had smoother surfaces. Immunohistochemistry revealed significant increases in the numbers of Caspase-3, interleukin-1β, matrix metalloprotease (MMP)-9, and MMP-13 positive cells, and few aggrecan positive cells, in treated p21–/– than in treated WT samples. Moreover, the number of TUNEL positive cells markedly increased in p21–/– condyles affected by mechanical stress. Conclusion: Our findings indicate that p21 in chondrocytes contributes to regulate matrix synthesis via the control of aggrecan and MMP-13 expression under mechanical stress. Thus, p21 might regulate the pathogenesis of osteoarthritis in the TMJ. |
掲載誌名 |
Clinical and Experimental Dental Research
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ISSN | 20574347
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出版者 | Wiley
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巻 | 7
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号 | 3
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開始ページ | 313
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終了ページ | 322
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発行日 | 2021-02-10
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備考 | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Tsendsuren Khurel-Ochirの学位論文として提出され,学位審査・授与の対象となっている。 |
権利情報 | This is an open access article under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第3493号
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学位記番号 | 甲口第471号
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学位授与年月日 | 2021-03-23
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学位名 |
博士(歯学)
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学位授与機関 |
徳島大学
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部局 |
歯学系
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