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ID 116060
Title Alternative
変形性顎関節症の進展におけるp21の免疫調節作用
Author
KHUREL-OCHIR, TSENDSUREN Tokushima University
Keywords
p21
chondrocytes
temporomandibular joint
osteoarthritis
metalloprotease
chondrocyte
Content Type
Thesis or Dissertation
Description
Objective: We aimed to identify the immunoregulatory role of the cyclin-dependent kinase inhibitor p21 in the homeostasis of mandibular condylar cartilage affected by mechanical stress.
Materials and Methods: Ten C57BL/6 wild-type (WT) and ten p21–/– mice aged 8 weeks were divided into the untreated and treated groups. In the treated groups, mechanical stress was applied to the temporomandibular joint (TMJ) through forced mouth opening for 3 h/day for 7 days. The dissected TMJs were assessed using micro-CT, histology, and immunohistochemistry.
Results: Treated p21–/– condyles with mechanical stress revealed more severe subchondral bone destruction, with thinner cartilage layers and smaller proteoglycan area relative to treated WT condyles; untreated WT and p21–/– condyles had smoother surfaces. Immunohistochemistry revealed significant increases in the numbers of Caspase-3, interleukin-1β, matrix metalloprotease (MMP)-9, and MMP-13 positive cells, and few aggrecan positive cells, in treated p21–/– than in treated WT samples. Moreover, the number of TUNEL positive cells markedly increased in p21–/– condyles affected by mechanical stress.
Conclusion: Our findings indicate that p21 in chondrocytes contributes to regulate matrix synthesis via the control of aggrecan and MMP-13 expression under mechanical stress. Thus, p21 might regulate the pathogenesis of osteoarthritis in the TMJ.
Journal Title
Clinical and Experimental Dental Research
ISSN
20574347
Publisher
Wiley
Volume
7
Issue
3
Start Page
313
End Page
322
Published Date
2021-02-10
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Tsendsuren Khurel-Ochirの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open access article under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3493号
Diploma Number
甲口第471号
Granted Date
2021-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences