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ID 116627
Author
Mizuno, Takahiko Tokushima University|JST
Oe, R. Tokushima University
Koresawa, H. Tokushima University
Yamamoto, Hirotsugu JST|Tokushima University|Utsunomiya University KAKEN Search Researchers
Content Type
Journal Article
Description
Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust to photobleaching. However, since most FLIMs are based on point-to-point measurements, mechanical scanning of a focal spot is needed for forming an image, which hampers rapid imaging. Here, we demonstrate scan-less full-field FLIM based on a one-to-one correspondence between two-dimensional (2D) image pixels and frequency-multiplexed radio frequency (RF) signals. A vast number of dual-comb optical beats between dual optical frequency combs are effectively adopted for 2D spectral mapping and high-density frequency multiplexing in the RF region. Bimodal images of fluorescence amplitude and lifetime are obtained with high quantitativeness from amplitude and phase spectra of fluorescence RF comb modes without the need for mechanical scanning. The parallelized FLIM will be useful for rapid quantitative fluorescence imaging in life science.
Journal Title
Science Advances
ISSN
23752548
Publisher
American Association for the Advancement of Science
Volume
7
Issue
1
Start Page
eabd2102
Published Date
2021-01-01
Rights
© 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC) (https://creativecommons.org/licenses/by-nc/4.0/).
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language
eng
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departments
Science and Technology
Institute of Post-LED Photonics