Matsumoto, Natsuki Kureha Special Laboratory Co., Ltd.|Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Hemmi, Akihiro Department of Pathology, Nihon University, Nerima Hikarigaoka Hospital
Yamato, Hideyuki Kureha Special Laboratory Co., Ltd.
Ohnishi, Ritsuko Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School
Segawa, Hiroko Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ohno, Shinichi Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
Miyamoto, Ken-ichi Department of Molecular Nutrition, Institution of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
in vivo cryotechnique
type II Na-Pi transporter
The “in vivo cryotechnique” (IVCT) is a new method of morphological analysis which has the advantage of freezing tissues in living animals without stopping their blood circulation. The purpose of this study was to investigate the effect of parathyroid hormone (PTH) on renal type II Na-Pi transporters (NaPi-IIa and NaPi-IIc) and ”cryobiopsy” (CB) using special cryoforceps as a simple method of the IVCT. The kidney tissues were biopsied at various time points after PTH administration by CB using liquid nitrogen as the cryogen. By hematoxylin-eosin (HE) staining the kidney tissues, well-frozen areas without visible ice crystals were obtained in the tissue surface areas, and the brush border membrane (BBM) of proximal tubules was well preserved at a light microscopic level. Immunohistochemical evaluation showed that PTH downregulated NaPi-IIa and NaPi-IIc at the BBM, being controlled by a different mechanism. In this method, the PTHinduced internalization of NaPi-IIc from microvilli to subapical compartments was not observed in the tissue preparations. NaPi-IIc protein appears to be degraded in microvilli of the proximal tubular cells after the injection of PTH. We suggest that CB using liquid nitrogen is useful to investigate renal type II Na-Pi transporters at the light microscopic level.
The journal of medical investigation : JMI
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
LID201112272002.pdf 1.09 MB