ID 113298
Author
Hirata, Yohko Tokushima University
Hosaka, Toshio Tokushima University
Le, Chung T.K. Tokushima University
Jambaldorj, Bayasgalan Tokushima University
Teshigawara, Kiyoshi Tokushima University
Keywords
Vimentin
GLUT4
IRAP
Content Type
Journal Article
Description
Insulin-responsive aminopeptidase (IRAP) and GLUT4 are two major cargo proteins of GLUT4 storage vesicles (GSVs) that are translocated from a postendosomal storage compartment to the plasma membrane (PM) in response to insulin. The cytoplasmic region of IRAP is reportedly involved in retention of GSVs. In this study, vimentin was identified using the cytoplasmic domain of IRAP as bait. The validity of this interaction was confirmed by pull-down assays and immunoprecipitation in 3T3-L1 adipocytes. In addition, it was shown that GLUT4 translocation to the PM by insulin was decreased in vimentin-depleted adipocytes, presumably due to dispersing GSVs away from the cytoskeleton. These findings suggest that the IRAP binding protein, vimentin, plays an important role in retention of GSVs.
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006291X
NCID
AA00564395
Publisher
Elsevier
Volume
405
Issue
1
Start Page
96
End Page
101
Published Date
2011-01-07
Remark
本論文は,著者Yohko Hirataの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences
Medical Sciences