ID 113896
Author
Okuda, Tomoyo Kyoto University
Kikukawa, Hiroshi Gifu University
Ando, Akinori Kyoto University
Kishino, Shigenobu Kyoto University
Izumi, Yoshihiro Kyushu University
Bamba, Takeshi Kyushu University
Shima, Jun Ryukoku University
Ogawa, Jun Kyoto University
Keywords
Mortierella alpina
Oleic acid
Linoleic acid
Fatty acid desaturase
RNAi
Content Type
Journal Article
Description
The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76 g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain.
Journal Title
Bioresource Technology
ISSN
18732976
09608524
NCID
AA11522750
AA10838373
Publisher
Elsevier
Volume
245
Issue
B
Start Page
1610
End Page
1615
Published Date
2017-06-19
Rights
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry