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ID 113990
Author
Itamochi, Shinya Osaka University
Hashimoto, Yoshihiro Osaka University
Keywords
Postmenopausal osteoporosis
Low-magnitude high-frequency vibration
Parathyroid hormone
Microcomputed tomography
micro-computed tomography
Infrared microspectroscopy
Nanoindentation testing
Content Type
Journal Article
Description
This study was designed to determine the effectiveness of whole-body vibration (WBV) and intermittent parathyroid hormone (iPTH) in combination against estrogen deficiency-induced osteoporosis. Female C57BL/6J mice were bilaterally ovariectomized (OVX, n = 40) or sham-operated (sham-OVX, n = 8) at 9 weeks of age. Two weeks later, the OVX mice were randomly divided into four groups (n = 10 each): the control group (c-OVX) and groups treated with iPTH (p-OVX), WBV (w-OVX) and both (pw-OVX). The p-OVX and pw-OVX groups were given human PTH (1–34) at a dose of 30 μg/kg/day. The w-OVX and pw-OVX groups were exposed to WBV at an acceleration of 0.3 g and 45 Hz for 20 min/day. All mice were euthanized after the 18-day treatment, and the left tibiae were harvested. The proximal metaphyseal region was μCT-scanned, and its cortical bone cross-section was analyzed by Fourier transform infrared microspectroscopy and nanoindentation testing. A single application of iPTH or WBV to OVX mice had no effect on bone structure or material properties of cortical bone, which were compromised in comparison with those in sham-OVX mice. The combination of iPTH and WBV improved trabecular bone volume, thickness and connectivity in OVX mice. Although the combined treatment failed to improve cortical bone structure, its mineral maturity and hardness restored to the levels observed in sham-OVX mice. There was no evidence of interaction between the two treatments, and the combined effects seemed to be additive. These results suggest combining WBV with iPTH has potential for treating postmenopausal osteoporosis.
Journal Title
Calcified Tissue International
ISSN
0171967X
14320827
NCID
AA00141085
Publisher
Springer US
Volume
98
Issue
5
Start Page
520
End Page
529
Published Date
2016-01-08
Remark
This is a post-peer-review, pre-copyedit version of an article published in Calcified Tissue International. The final authenticated version is available online at: https://doi.org/10.1007/s00223-015-0104-4.
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DOI (Published Version)
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language
eng
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departments
Science and Technology