ID 113977
Title Alternative
CXCR3+ macrophage in Sjögren's syndrome
Author
Nakashiro, Koh-ichi Ehime University
Keywords
CXCR3
lip salivary gland
macrophage
Sjögren's syndrome
salivary gland
LSG
Content Type
Journal Article
Description
Background: Mechanisms underlying immune cells' recruitment and activation into the inflammatory lesions of lip salivary glands (LSGs) from primary Sjögren's syndrome (pSS) patients are incompletely understood. Chemokines play pivotal roles in these processes, so we investigated the clinical significance of chemokine receptor CXCR3 and its ligands in the autoimmune lesions of pSS.
Methods: We histologically determined the grade of LSG samples from 22 pSS patients and subjected the samples to immunofluorescence analysis to determine the expressions of CXCR3 and its ligands: CXCL9, CXCL10, and CXCL11. To identify the immune cells expressing CXCR3 in the LSGs, we performed double immunofluorescence analysis using antibodies against CD3 (pan-T cells), CD80 (M1 macrophages), CD163 (M2 macrophage), and CD123 (plasmacytoid dendritic cells: pDCs). The relationship between the grade of lymphocytic infiltration and the number of positively stained cells was analyzed by Spearman's rank correlation test.
Results: The expressions of CXCL9 and CXCL10 showed particularly intense staining in the LSG samples' ductal cells. The CXCR3 expression was detected mainly in CD80+ and CD163+ macrophages. The number of CXCR3+CD163+ macrophages inversely correlated with the LSG inflammatory lesions' severity (rs= −0.777, p<0.001).
Conclusions: Our results suggest that the enhanced production of CXCL9 and CXCL10 from ductal cells results in the CXCR3+ macrophages' migration. There was an inverse correlation between these two parameters: i.e., the number of CXCR3+CD163+ macrophages decreased as the lymphocytic infiltration grade increased. Although CXCR3 is expressed in all of the innate immune cells, CXCR3+CD163+ M2 macrophages may contribute to the anti-inflammatory functions in pSS lesions.
Journal Title
Journal of Oral Pathology & Medicine
ISSN
09042512
16000714
NCID
AA10715555
Publisher
John Wiley & Sons A/S
Volume
47
Issue
7
Start Page
710
End Page
718
Published Date
2018-06-21
Remark
This is the peer reviewed version of the following article: Aota, K, Yamanoi, T, Kani, K, Nakashiro, K‐I, Ishimaru, N, Azuma, M. Inverse correlation between the number of CXCR3+ macrophages and the severity of inflammatory lesions in Sjögren's syndrome salivary glands: A pilot study. J Oral Pathol Med. 2018; 47: 710– 718, which has been published in final form at https://doi.org/10.1111/jop.12756. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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DOI (Published Version)
URL ( Publisher's Version )
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language
eng
TextVersion
Author
departments
University Hospital
Oral Sciences