ID 109875
Title Transcription
シニクコウ シンシュツエキ チュウ ノ バイオマーカー オ モチイタ シシュウビョウ シンダン
Title Alternative
Diagnosis of Periodontal Diseases using Biomarkers in Gingival Crevicular Fluid
Author
Kido, Jun-ichi Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Bando, Mika Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Inagaki, Yuji Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ikuta, Takahisa Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
Kajiura, Yukari Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School KAKEN Search Researchers
Nakajima, Yukiko Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Sakamoto, Eijiro Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory
Mihara, Chie Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Ninomiya, Masami Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nishikawa, Yasufumi Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
Lew, Jung-Hwan Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School
Naruishi, Koji Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nagata, Toshihiko Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
歯周病診断
バイオマーカー
歯肉溝滲出液
Content Type
Journal Article
Description
Periodontal diseases cause an inflammation and degradation of periodontal tissues and missing of teeth. The incidence rate of periodontal diseases is high in middle-aged and elderly people.
A reasonable diagnosis of periodontal diseases is very important to keep teeth, however, conventional examinations of periodontal diseases is not necessarily exact and objective. Gingival crevicular fluid (GCF) is an exudate secreted from periodontal tissues and contains many components including proteolytic enzymes, inflammatory cytokines, blood-associated proteins, cellular and bacterial fragments. Because some proteins in GCF are related to inflammation, tissue degradation and bone metabolism, those proteins have been studying as a diagnostic marker of periodontal diseases. GCF is noninvasively collected using a sterile paper strip and biomarkers are determined using enzyme-linked immunosorbent assay (ELISA) and enzyme activity assay. We identified calprotectin, an inflammationrelated protein, in GCF and calprotectin level in GCF from periodontitis sites was significantly higher than that of healthy control. Calprotectin level in GCF was positively correlated to gingival index and other biomarkers and decreased by periodontal treatments. Resistin is an adipocytokine and its level increases in some inflammatory diseases. Resistin level in GCF from periodontitis sites was high compared to the level of healthy control samples. Procollagen type I C-terminal peptide (PICP) is a biomarker for bone metabolism and its level was high in GCF collected from periodontitis sites. These results suggested that calprotectin, resistin and PICP are useful biomarkers for periodontal diseases. On the other hand, we showed that glycated albumin (GA), a marker of diabetes mellitus (DM), was contained in GCF and GA level in GCF from DM patients was significantly higher than that of non-DM individuals.
Components in GCF may be biomarkers of systemic diseases as well as periodontal diseases and their determination will be useful diagnostic examination of some diseases. Recently, we have been studying the determining system of GCF calprotectin, including microchip ELISA, surface plasmon resonance assay and immuno-chromatography assay. When GCF biomarkers are determined using the determining systems, we will simply, exactly and objectively diagnose periodontal diseases at our dental offices.
Journal Title
Journal of Oral Health and Biosciences
ISSN
21887888
NCID
AA12713630
Publisher
四国歯学会
Volume
29
Issue
1
Start Page
13
End Page
20
Sort Key
13
Published Date
2016-06-30
EDB ID
FullText File
language
jpn
TextVersion
Publisher
departments
Oral Sciences
University Hospital