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ID 114776
Author
Tanikami, Yuki Tokushima University
Tagami, Takuma Tokushima University
Sakamoto, Mayu Tokushima University
Keywords
Acid dissociation constant
Flavin analogues
Instable substances
Capillary zone electrophoresis
Content Type
Journal Article
Description
Acid dissociation constants (pKa) of 9 kinds of flavin analogues as molecular catalyst candidates were determined by CZE. Although some of the analogues are instable and degradable under the light-exposure or in alkaline aqueous solutions, the effective electrophoretic mobility of the flavin analogue of interest has been measured with the residual substance. The pKa values of the flavin analogues were analyzed through the changes in the effective electrophoretic mobility with varying pH of the separation buffer. One or two steps pKa values were determined by the analysis. One of the degraded species from the flavin analogues, lumichrome, was also detected in the CZE analysis, and its pKa values were also determined. While coexisting impurities generated over the storage conditions were found in some analogues, the pKa values of the target analogues were successfully determined with the help of the CZE separations. A pressure-assisted CZE was utilized for the determination or the estimation of the pKa values of such analogues as possessing carboxylic acid moiety.
Journal Title
Electrophoresis
ISSN
15222683
01730835
NCID
AA00179882
Publisher
WILEY-VCH
Volume
41
Issue
15
Start Page
1316
End Page
1325
Published Date
2020-05-09
Rights
This is the peer reviewed version of the following article: Tanikami, Y., Tagami, T., Sakamoto, M., Arakawa, Y., Mizuguchi, H., Imada, Y. and Takayanagi, T. (2020), Determination of acid dissociation constants of flavin analogues by capillary zone electrophoresis. ELECTROPHORESIS, 41: 1316-1325., which has been published in final form at https://doi.org/10.1002/elps.202000066. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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language
eng
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departments
Science and Technology