number of access : ?
number of downloads : ?
ID 115102
Title Alternative
AMINO ACID PROFILES IN MICE WITH NASH
Author
Iida, Ayaka Kanagawa University of Human Services|Fukuoka Women’s University
Kuranuki, Sachi Kanagawa University of Human Services
Yamamoto, Ryoko Hirosaki University
Uchida, Masaya Ariake College
Ohta, Masanori Fukuoka Women’s University
Masaki, Takayuki Oita University
Seike, Masataka Oita University
Nakamura, Tsuyoshi Fukuoka Women’s University
Keywords
amino acid metabolism disorder
diabetes
hyperlipidemia
non-alcoholic fatty liver disease
non-alcoholic steatohepatitis
Content Type
Journal Article
Description
The changes in free amino acid (AA) levels in blood during the progression from non-alcoholic steatohepatitis (NASH) to hepatocellular carcinoma (HCC) are unclear. We investigated serum AA levels, along with biochemical and histological events, in a mouse model of NASH. We induced NASH in male C57BL/6J mice with a streptozotocin injection and high-fat diet after 4 weeks of age (STAM group). We chronologically (6, 8, 10, 12, and 16 weeks, n=4–12 mice/group) evaluated the progression from steatohepatitis to HCC by biochemical and histological analyses. The serum AA levels were determined using an AA analyzer. Serum aspartate aminotransferase and alanine aminotransferase levels were higher in the STAM group than in the normal group (non-NASH-induced mice). Histological analysis revealed that STAM mice had fatty liver, NASH, and fibrosis at 6, 8, and 10 weeks, respectively. Moreover, the mice exhibited fibrosis and HCC at 16 weeks. The serum branched-chain AA levels were higher in the STAM group than in the normal group, especially at 8 and 10 weeks. The Fischer ratio decreased at 16 weeks in the STAM group, with increasing aromatic AA levels. These results suggested that this model sequentially depicts the development of fatty liver, NASH, cirrhosis, HCC, and AA metabolism disorders within a short experimental period. Additionally, serum amyloid A was suggested to be a useful inflammation biomarker associated with NASH. We believe that the STAM model will be useful for studying AA metabolism and/or pharmacological effects in NASH.
Journal Title
Experimental Animals
ISSN
13411357
18817122
NCID
AA11032321
Publisher
Japanese Association for Laboratory Animal Science
Volume
68
Issue
4
Start Page
417
End Page
428
Published Date
2019
Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/4.0/>.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences