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ID 117221
Author
Morimoto, Yuki Tokushima University
Suzuki, Akihiro Tokushima University
Nakamura , Satoko Kagawa Prefectural Central Hospital
Tsutsui, Akemi Kagawa Prefectural Central Hospital
Takaguchi, Koichi Kagawa Prefectural Central Hospital
Keywords
second-harmonic generation microscopy
non-alcoholic fatty liver disease
non-alcoholic steatohepatitis
fibrosis
Content Type
Journal Article
Description
Non-alcoholic fatty liver disease (NAFLD) is associated with the chronic progression of fibrosis. In general, the progression of liver fibrosis is determined by a histopathological assessment with a collagen-stained section; however, the ultra-early stage of liver fibrosis is challenging to identify because of the low sensitivity in the collagen-selective staining method. In the present study, we demonstrate the feasibility of second-harmonic generation (SHG) microscopy in the histopathological diagnosis of the liver of NAFLD patients for the quantitative assessment of the ultra-early stage of fibrosis. We investigated four representative NAFLD patients with early stages of fibrosis. SHG microscopy visualised well-matured fibrotic structures and early fibrosis diffusely involving liver tissues, whereas early fibrosis is challenging to be identified by conventional histopathological methods. Furthermore, the SHG emission directionality analysis revealed the maturation of each collagen fibre of each patient. As a result, SHG microscopy is feasible for assessing liver fibrosis on NAFLD patients, including the ultra-early stage of liver fibrosis that is difficult to diagnose by the conventional histopathological method. The assessment method of the ultra-early fibrosis by using SHG microscopy may serve as a crucial means for pathological, clinical, and prognostic diagnosis of NAFLD patients.
Journal Title
International Journal of Molecular Sciences
ISSN
14220067
Publisher
MDPI
Volume
23
Issue
6
Start Page
3357
Published Date
2022-03-20
Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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DOI (Published Version)
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language
eng
TextVersion
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departments
Science and Technology
Institute of Post-LED Photonics
Medical Sciences