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ID 113337
Title Alternative
ポドサイト傷害マウスモデルを用いた腎症進展に対する細胞外リン濃度の役割
Role of Extracellular Pi Levels on Kidney Disease Progression in a Podocyte Injury
Author
Maeda, Akira Tokushima University|Chugai Pharmaceutical Co., Ltd.
Fukushima, Naoshi Chugai Pharmaceutical Co., Ltd.
Horiba, Naoshi Chugai Pharmaceutical Co., Ltd.
Keywords
Focal segmental glomerulosclerosis
Kidney disease
Phosphate
Phosphate binder
Renal function
Content Type
Thesis or Dissertation
Description
Background: Hyperphosphatemia is a major accelerator of complications in chronic kidney disease and dialysis, and phosphate (Pi) binders have been shown to regulate extracellular Pi levels. Research on hyperphosphatemia in mouse models is scarce, and few models display hyperphosphatemia induced by glomerular injury, despite its relevance to human glomerular disease conditions. In this study, we investigated the involvement of hyperphosphatemia in kidney disease progression using a mouse model in which hyperphosphatemia is induced by focal segmental glomerulosclerosis (FSGS). Methods: We established the NEP25 mouse model in which FSGS-hyperphosphatemia is induced by podocyte injury and evaluated the effect of a Pi binder, sevelamer. Results: After disease induction, we confirmed a gradual increase in serum Pi accompanied by reduced renal function and observed increases in serum FGF23 and PTH. Treatment with sevelamer significantly reduced serum Pi and urinary Pi fractional excretion and suppressed increases in serum FGF23 and PTH. A high dose improved serum creatinine and tubular injury markers, and pathological analysis confirmed amelioration of glomerular and tubular damage. Gene expression and marker analysis suggested protective effects on tubular epithelial cells in the diseased kidney. Compared to disease control, NEP25 mice treated with sevelamer retained their mRNA expression of Klotho, a known FGF23 co-receptor and renoprotective factor. Conclusions: Hyperphosphatemia caused by renal function decline was observed in a FSGS-induced NEP25 mouse model. Studies using this model showed that Pi regulation had a positive impact on kidney disease progression, and notably on tubular epithelial cell injury, which indicates the importance of Pi regulation in the treatment of kidney disease progression.
Journal Title
Nephron
ISSN
16608151
22353186
NCID
AA1278405X
Publisher
Karger
Volume
142
Issue
2
Start Page
135
End Page
146
Published Date
2019-02-07
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Akira Maedaの学位論文として提出され,学位審査・授与の対象となっている。
Rights
© 2019 S. Karger AG, Basel
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3275号
Diploma Number
甲栄第264号
Granted Date
2019-03-14
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences