ID | 116345 |
Title Alternative | 細胞診における探針エレクトロスプレーイオン化質量分析の有用性 : 肺癌培養細胞での検討
PESI-MS for Diagnostic Cytology
|
Author |
Morimoto, Yuki
Tokushima University
Ichimura-Shimizu, Mayuko
Tokushima University
Tokushima University Educator and Researcher Directory
Ogawa, Hirohisa
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Kobayashi, Tomoko
Tokushima University
Sumida, Satoshi
Tokushima University
Kakimoto, Takumi
Tokushima University
Cheng, Chunmei
Novo Nordisk Research Centre China
|
Keywords | Mass spectrometry
Rapid cytological diagnosis
Probe electrospray ionization mass spectrometry
Lung cytopathology
|
Content Type |
Thesis or Dissertation
|
Description | Objectives: Cytology and histology are 2 indispensable diagnostic tools for cancer diagnosis, which are rapidly increasing in importance with aging populations. We applied mass spectrometry (MS) as a rapid approach for swiftly acquiring nonmorphological information of interested cells. Conventional MS, which primarily rely on promoting ionization by pre-applying a matrix to cells, has the drawback of time-consuming both on data acquisition and analysis. As an emerging method, probe electrospray ionization-MS (PESI-MS) with a dedicated probe is capable to pierce sample and measure specimen in small amounts, either liquid or solid, without the requirement for sample pretreatment. Furthermore, PESI-MS is timesaving compared to the conventional MS. Herein, we investigated the capability of PESI-MS to characterize the cell types derived from the respiratory tract of human tissues. Study Design: PESI-MS analyses with DPiMS-2020 were performed on various type of cultured cells including 5 lung squamous cell carcinomas, 5 lung adenocarcinomas, 5 small-cell carcinomas, 4 malignant mesotheliomas, and 2 normal controls. Results: Several characteristic peaks were detected at around m/z 200 and 800 that were common in all samples. As expected, partial least squares-discriminant analysis of PESI-MS data distinguished the cancer cell types from normal control cells. Moreover, distinct clusters divided squamous cell carcinoma from adenocarcinoma. Conclusion: PESI-MS presented a promising potential as a novel diagnostic modality for swiftly acquiring specific cytological information.
|
Journal Title |
Acta Cytologica
|
ISSN | 00015547
19382650
|
NCID | AA00044384
AA12780809
|
Publisher | Karger
|
Volume | 65
|
Issue | 5
|
Start Page | 430
|
End Page | 439
|
Published Date | 2021-06-07
|
Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Yuki Morimotoの学位論文として提出され,学位審査・授与の対象となっている。 |
Rights | © 2021 S. Karger AG, Basel
|
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
|
TextVersion |
ETD
|
MEXT report number | 甲第3535号
|
Diploma Number | 甲医第1502号
|
Granted Date | 2021-09-09
|
Degree Name |
Doctor of Medical Science
|
Grantor |
Tokushima University
|
departments |
University Hospital
Medical Sciences
|