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ID 117376
Title Alternative
αカテニンの適切な張力感受性は上皮スフェロイドの球形への形態形成を確実にする
Tension-sensitivity of α-catenin on morphogenesis
Author
Nishimura, Ryosuke Tokushima University
Kato, Kagayaki National Institutes of Natural Sciences
Saida, Misako National Institute for Basic Biology
Kamei, Yasuhiro National Institute for Basic Biology|The Graduate University for Advanced Studies (SOKENDAI)
Takeda, Masahiro RIKEN
Miyoshi, Hiromi RIKEN|Tokyo Metropolitan University
Yamagata, Yutaka RIKEN
Amano, Yu Kwansei Gakuin University
Keywords
αカテニン
ビンキュリン
細胞間接着
上皮形態形成
メカノトランスダクション
α-catenin
vinculin
adherens junction
morphogenesis
mechanotransduction
Content Type
Thesis or Dissertation
Description
The adherens junction (AJ) is an actin filament-anchoring junction. It plays a central role in epithelial morphogenesis through cadherin-based recognition and adhesion among cells. The stability and plasticity of AJs are required for the morphogenesis. An actin-binding α-catenin is an essential component of the cadherin-catenin complex and functions as a tension transducer that changes its conformation and induces AJ development in response to tension. Despite much progress in understanding molecular mechanisms of tension sensitivity of α-catenin, its significance on epithelial morphogenesis is still unknown. Here we show that the tension sensitivity of α-catenin is essential for epithelial cells to form round spheroids through proper multicellular rearrangement. Using a novel in vitro suspension culture model, we found that epithelial cells form round spheroids even from rectangular-shaped cell masses with high aspect ratios without using high tension and that increased tension sensitivity of α-catenin affected this morphogenesis. Analyses of AJ formation and cellular tracking during rounding morphogenesis showed cellular rearrangement, probably through AJ remodeling. The rearrangement occurs at the cell mass level, but not single-cell level. Hypersensitive α-catenin mutant-expressing cells did not show cellular rearrangement at the cell mass level, suggesting that the appropriate tension sensitivity of α-catenin is crucial for the coordinated round morphogenesis.
Journal Title
Cell Structure and Function
ISSN
13473700
03867196
NCID
AA0060007X
Publisher
Japan Society for Cell Biology
Volume
47
Issue
2
Start Page
55
End Page
73
Published Date
2022-06-22
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Ryosuke Nishimuraの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open access article distributed under the terms of the Creative Commons BY (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3644号
Diploma Number
甲医第1537号
Granted Date
2022-7-28
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences