ID | 117376 |
Title Alternative | αカテニンの適切な張力感受性は上皮スフェロイドの球形への形態形成を確実にする
Tension-sensitivity of α-catenin on morphogenesis
|
Author |
Nishimura, Ryosuke
Tokushima University
Kato, Kagayaki
National Institutes of Natural Sciences
Saida, Misako
National Institute for Basic Biology
Kamei, Yasuhiro
National Institute for Basic Biology|The Graduate University for Advanced Studies (SOKENDAI)
Takeda, Masahiro
RIKEN
Miyoshi, Hiromi
RIKEN|Tokyo Metropolitan University
Yamagata, Yutaka
RIKEN
Amano, Yu
Kwansei Gakuin University
Yonemura, Shigenobu
Tokushima University|RIKEN
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
|
Keywords | αカテニン
ビンキュリン
細胞間接着
上皮形態形成
メカノトランスダクション
α-catenin
vinculin
adherens junction
morphogenesis
mechanotransduction
|
Content Type |
Thesis or Dissertation
|
Description | The adherens junction (AJ) is an actin filament-anchoring junction. It plays a central role in epithelial morphogenesis through cadherin-based recognition and adhesion among cells. The stability and plasticity of AJs are required for the morphogenesis. An actin-binding α-catenin is an essential component of the cadherin-catenin complex and functions as a tension transducer that changes its conformation and induces AJ development in response to tension. Despite much progress in understanding molecular mechanisms of tension sensitivity of α-catenin, its significance on epithelial morphogenesis is still unknown. Here we show that the tension sensitivity of α-catenin is essential for epithelial cells to form round spheroids through proper multicellular rearrangement. Using a novel in vitro suspension culture model, we found that epithelial cells form round spheroids even from rectangular-shaped cell masses with high aspect ratios without using high tension and that increased tension sensitivity of α-catenin affected this morphogenesis. Analyses of AJ formation and cellular tracking during rounding morphogenesis showed cellular rearrangement, probably through AJ remodeling. The rearrangement occurs at the cell mass level, but not single-cell level. Hypersensitive α-catenin mutant-expressing cells did not show cellular rearrangement at the cell mass level, suggesting that the appropriate tension sensitivity of α-catenin is crucial for the coordinated round morphogenesis.
|
Journal Title |
Cell Structure and Function
|
ISSN | 13473700
03867196
|
NCID | AA0060007X
|
Publisher | Japan Society for Cell Biology
|
Volume | 47
|
Issue | 2
|
Start Page | 55
|
End Page | 73
|
Published Date | 2022-06-22
|
Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Ryosuke Nishimuraの学位論文として提出され,学位審査・授与の対象となっている。 |
Rights | This is an open access article distributed under the terms of the Creative Commons BY (Attribution) License (https://creativecommons.org/licenses/by/4.0/legalcode), which permits the unrestricted distribution, reproduction and use of the article provided the original source and authors are credited.
|
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
|
TextVersion |
ETD
|
MEXT report number | 甲第3644号
|
Diploma Number | 甲医第1537号
|
Granted Date | 2022-7-28
|
Degree Name |
Doctor of Medical Science
|
Grantor |
Tokushima University
|
departments |
Medical Sciences
|