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ID 112841
Title Alternative
リアルタイムイメージングによる栄養チューブ内における流動食残渣と微生物増殖の検出
Author
Yamaoka, Ippei Otsuka Pharmaceutical Factory, Inc.
Kagawa, Tomohiro Otsuka Pharmaceutical Factory, Inc.
Mizugai, Kazuya Otsuka Pharmaceutical Factory, Inc.
Ebisu, Goro Otsuka Pharmaceutical Factory, Inc.
Keywords
bacteria
biofilms
bioluminescence
enteral nutrition
feeding tubes
fluorescence
Content Type
Thesis or Dissertation
Description
Background:
Enteral nutrition (EN) residues that persist in feeding tubes provide substrates for microorganisms to proliferate and occlude the tubes. Visible EN residues in tubes are easily identified, but smaller residues can persist. We developed a new imaging technique to visualize EN residues and proliferation of microorganisms in feeding tubes.
Materials and Methods:
Feeding tubes containing EN labelled with fluorescent dye and EN with or without various types or amounts of thickeners were flushed once with water and then the tubes were seeded with Pseudomonas aeruginosa Xen5 with recombinant luciferase DNA. Because EN fluoresces intrinsically, EN in the feeding tubes without fluorescent dye was repeatedly flushed until the intrinsic fluorescence levels reached background levels. Fluorescent images of EN residues and bioluminescent images of microorganisms were acquired using an optical imaging system.
Results:
Fluorescence images showed that the amount of EN residues increased at various sites in tubes depending on EN viscosity and the thickening agent, and bioluminescence images showed that microorganism proliferation was associated with a commensurate increase in EN residues. The intrinsic fluorescence of EN also enabled the detection of EN residues in tubes even in the absence of fluorescence dye. Higher EN viscosity required more flushes to reach undetectable levels.
Conclusion:
EN residues and microorganism proliferation in enteral feeding tubes were detected on fluorescence and bioluminescence images, respectively. This simplified approach allowed the real-time visualization of EN residues and microorganisms in feeding tubes.
Journal Title
Nutrtion in Clinical Practice
ISSN
19412452
Publisher
Wiley|American Society for Parenteral and Enteral Nutrition
Volume
32
Issue
2
Start Page
282
End Page
287
Published Date
2016-11-04
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Ippei Yamaokaの学位論文として提出され, 学位審査・授与の対象となっている。
This is the peer reviewed version of the following article: Yamaoka, I. , Kagawa, T. , Mizugai, K. and Ebisu, G. (2017), Detecting Enteral Nutrition Residues and Microorganism Proliferation in Feeding Tubes via Real‐Time Imaging. Nutrition in Clinical Practice, 32: 282-287, which has been published in final form at https://doi.org/10.1177/0884533616675189. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
乙第2104号
Diploma Number
乙栄第95号
Granted Date
2018-09-27
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University