ID 111121
著者
Nuka, Erika Tokushima University
Tomono, Susumu University of Shizuoka
Ishisaka, Akari University of Hyogo
Kato, Yoji University of Hyogo
Miyoshi, Noriyuki University of Shizuoka
資料タイプ
学術雑誌論文
抄録
Lipid peroxidation products react with cellular molecules, such as DNA bases, to form covalent adducts, which are associated with aging and disease processes. Since lipid peroxidation is a complex process and occurs in multiple stages, there might be yet unknown reaction pathways. Here, we analyzed comprehensively 2′-deoxyguanosine (dG) adducts with oxidized arachidonic acid using liquid chromatography–tandem mass spectrometry and found the formation of 7-(2-oxo-hexyl)-etheno-dG as one of the major unidentified adducts. The formation of this adduct was reproduced in the reaction of dG with 2-octenal and predominantly with 4-oxo-2-octenal (OOE). We also found that other 2-alkenals (with five or more carbons) generate corresponding 4-oxo-2-alkenal-type adducts. Importantly, it was found that transition metals enhanced the oxidation of C4-position of 2-octenal, leading to the formation of OOE-dG adduct. These findings demonstrated a new pathway for the formation of 4-oxo-2-alkenals during lipid peroxidation and might provide a mechanism for metal-catalyzed genotoxicity.
掲載誌名
Bioscience, Biotechnology, and Biochemistry
ISSN
13476947
09168451
cat書誌ID
AA12018610
AA10824164
出版者
Taylor & Francis
80
10
開始ページ
2007
終了ページ
2013
発行日
2016-06-07
権利情報
© 2016 Japan Society for Bioscience, Biotechnology, and Agrochemistry. This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 07 Jun 2016, available online: http://www.tandfonline.com/10.1080/09168451.2016.1191334
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
医学系